Temporal expression of PDGF receptors and PDGF regulatory effects on osteoblastic cells in mineralizing cultures

Xiaohui Yu, Sung Chih Hsieh, Wei Bao, Dana T. Graves

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Platelet-derived growth factor (PDGF) is mitogenic and chemotactic for osteoblastic cells in vitro. It is expressed during osseous wound healing and stimulates formation of new bone in vivo. PDGF stimulates cells by binding to specific cell surface receptors. The purpose of this study was to examine the effects of PDGF on osteoblastic proliferation and differentiation in long- term mineralizing cultures. Utilizing Northern blot analysis, we found that continuous PDGF treatment increased histone expression, indicative of enhanced proliferation, but suppressed osteoblast differentiation, demonstrated by inhibition of alkaline phosphatase, type I collagen, and osteocalcin expression. The inhibitory effect of PDGF on the differentiated function of osteoblasts was further established by findings that PDGF significantly inhibited nodule formation. The expression of PDGF receptors varied at different stages of culture. PDGF receptor mRNA expression increased when the cells had achieved a mature phenotype, during the stage of matrix maturation, and then decreased. However, as demonstrated by thyroidine incorporation assays, the capacity of PDGF to stimulate DNA synthesis actually decreased during osteoblast maturation, as receptor expression increased. To investigate this apparent contradiction, tyrosyl phosphorylation and immunoblot assays were performed to assess changes in PDGF activation of their cognate receptors. The pattern of PDGF-induced tyrosyl phosphorylation remained relatively constant. This suggests that the diminished mitogenic activity of PDGF that occurs after osteoblast differentiation is regulated at a postreceptor level.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume272
Issue number5 41-5
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Platelet-Derived Growth Factor Receptors
Platelet-Derived Growth Factor
Osteoblasts
Phosphorylation
Assays
Osteocalcin
Cell Surface Receptors
Collagen Type I
Osteogenesis
Northern Blotting
Histones
Wound Healing
Alkaline Phosphatase
Bone
Chemical activation
Phenotype

Keywords

  • bone
  • differentiation
  • nodule formation
  • platelet-derived growth factor-induced tyrosyl phosphorylation
  • proliferation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Temporal expression of PDGF receptors and PDGF regulatory effects on osteoblastic cells in mineralizing cultures. / Yu, Xiaohui; Hsieh, Sung Chih; Bao, Wei; Graves, Dana T.

In: American Journal of Physiology - Cell Physiology, Vol. 272, No. 5 41-5, 1997.

Research output: Contribution to journalArticle

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