TEMPO-Oxidized Bacterial Cellulose Pellicle with Silver Nanoparticles for Wound Dressing

Chun Nan Wu, Shih Chang Fuh, Shin Ping Lin, Yen Yi Lin, Hung Yueh Chen, Jui Ming Liu, Kuan Chen Cheng

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Biocompatible bacterial cellulose pellicle (BCP) is a candidate for biomedical material such as wound dressing. However, due to lack of antibacterial activity, to grant BCP with the property is crucial for its biomedical application. In the present study, BCP was modified by 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation using TEMPO/NaClO/NaBr system at pH 10 to form TEMPO-oxidized BCP (TOBCP) with anionic C6 carboxylate groups. The TOBCP was subsequently ion-exchanged in AgNO3 solution and silver nanoparticles (AgNP) with diameter of ∼16.5 nm were in situ synthesized on TOBC nanofiber surfaces by thermal reduction without using a reducing agent. Field emission scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectra, Fourier transform infrared spectroscopy, and thermogravimetric analysis were carried out to confirm morphology and structure of the pellicles with AgNP. The AgNP continuously released Ag+ with a rate of 12.2%/day at 37 °C in 3 days. The TOBCP/AgNP exhibited high biocompatibility according to the result of in vitro cytotoxicity test (cell viability >95% after 48 h of incubation) and showed significant antibacterial activities of 100% and 99.2% against E. coli and S. aureus, respectively. Hence, the highly biocompatible and highly antibacterial TOBCP/AgNP prepared in the present study is a promising candidate for wound dressing.

Original languageEnglish
Pages (from-to)544-554
Number of pages11
JournalBiomacromolecules
Volume19
Issue number2
DOIs
Publication statusPublished - Feb 12 2018
Externally publishedYes

Fingerprint

Silver
Cellulose
Nanoparticles
Reducing Agents
Reducing agents
Cytotoxicity
Nanofibers
Photoelectrons
Biocompatibility
Field emission
Escherichia coli
Fourier transform infrared spectroscopy
Thermogravimetric analysis
Cells
Ions
X ray diffraction
X rays
Oxidation
Scanning electron microscopy
TEMPO

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

TEMPO-Oxidized Bacterial Cellulose Pellicle with Silver Nanoparticles for Wound Dressing. / Wu, Chun Nan; Fuh, Shih Chang; Lin, Shin Ping; Lin, Yen Yi; Chen, Hung Yueh; Liu, Jui Ming; Cheng, Kuan Chen.

In: Biomacromolecules, Vol. 19, No. 2, 12.02.2018, p. 544-554.

Research output: Contribution to journalArticle

Wu, Chun Nan ; Fuh, Shih Chang ; Lin, Shin Ping ; Lin, Yen Yi ; Chen, Hung Yueh ; Liu, Jui Ming ; Cheng, Kuan Chen. / TEMPO-Oxidized Bacterial Cellulose Pellicle with Silver Nanoparticles for Wound Dressing. In: Biomacromolecules. 2018 ; Vol. 19, No. 2. pp. 544-554.
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