Template supercoiling during ATP-dependent DNA helix tracking

Studies with simian virus 40 large tumor antigen

L. Yang, C. B. Jessee, K. Lau, H. Zhang, Leroy-Fong Liu

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Incubation of topologically relaxed plasmid DNA with simian virus 40 (SV40) large tumor antigen (T antigen), ATP, and eubacterial DNA topoisomerase I resulted in the formation of highly positively supercoiled DNA. Eukaryotic DNA topoisomerase I could not substitute for eubacterial DNA topoisomerase I in this reaction. Furthermore, the addition of eukaryotic topoisomerase I to a preincubated reaction mixture containing both T antigen and eubacterial topoisomerase I caused rapid relaxation of the positively supercoiled DNA. These results suggest that SV40 T antigen can introduce topoisomerase-relaxable supercoils into DNA in a reaction coupled to ATP hydrolysis. We interpret the observed T antigen supercoiling reaction in terms of a recently proposed twin-supercoiled-domain model that describes the mechanics of DNA helix-tracking processes. According to this model, positive and negative supercoils are generated ahead of and behind the moving SV40 T antigen, respectively. The preferential relaxation of negative supercoils by eubacterial DNA topoisomerase I explains the accumulation of positive supercoils in the DNA template. The supercoiling assay using DNA conformation-specific eubacterial DNA topoisomerase I may be of general use for the detection of ATP-dependent DNA helix-tracking proteins.

Original languageEnglish
Pages (from-to)6121-6125
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number16
Publication statusPublished - 1989
Externally publishedYes

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Type I DNA Topoisomerase
Simian virus 40
Neoplasm Antigens
Adenosine Triphosphate
DNA
Superhelical DNA
Nucleic Acid Conformation
Mechanics
Hydrolysis
Plasmids

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Template supercoiling during ATP-dependent DNA helix tracking : Studies with simian virus 40 large tumor antigen. / Yang, L.; Jessee, C. B.; Lau, K.; Zhang, H.; Liu, Leroy-Fong.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 16, 1989, p. 6121-6125.

Research output: Contribution to journalArticle

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