Temperature regulates the arrhythmogenic activity of pulmonary vein cardiomyocytes

Yi Jen Chen, Yao Chang Chen, Paul Chan, Cheng I. Lin, Shih Ann Chen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Temperature plays an important role in the electrophysiology of cardiomyocytes. Pulmonary veins (PVs) are known to initiate paroxysmal atrial fibrillation. The effects of temperature on the arrhythmogenic activity of rabbit single PV and atrial cardiomyocytes were assessed using the whole-cell clamp technique. PV cardiomyocytes had different beating rates at low (22-25°C), normal (38-39°C) and high (40-41°C) temperatures (0.9 ± 0.1, 3.2 ± 0.4, 6.4 ± 0.6 Hz, respectively; p <0.001). There were different action potential durations and incidences of delayed afterdepolarization in PV cardiomyocytes with pacemaker activity (31, 59, 63%; p <0.05), PV cardiomyocytes without pacemaker activity (16, 47, 60%; p <0.001), and atrial myocytes (0, 0, 21%; p <0.05). However, oscillatory afterpotentials were only found in PV cardiomyocytes with pacemaker activity at normal (50%) or high (68%) temperatures, but not at low temperatures (p <0.001). Both PV and atrial cardiomyocytes had larger transient inward currents and inward rectified currents at high temperatures. Additionally, PV cardiomyocytes with and without pacemaker activity had larger pacemaker currents at higher temperatures. This study demonstrated that PV cardiomyocytes have an increase in arrhythmogenic activity at high temperatures because of enhanced automaticity, induced triggered activity, or shortening of action potential duration.

Original languageEnglish
Pages (from-to)535-543
Number of pages9
JournalJournal of Biomedical Science
Volume10
Issue number5
DOIs
Publication statusPublished - 2003
Externally publishedYes

Fingerprint

Pulmonary Veins
Cardiac Myocytes
Pacemakers
Thermodynamic properties
Temperature
Action Potentials
Electrophysiology
Clamping devices
Atrial Fibrillation
Muscle Cells
Rabbits
Incidence

Keywords

  • Action potential
  • Atrial fibrillation
  • Triggered activity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Temperature regulates the arrhythmogenic activity of pulmonary vein cardiomyocytes. / Chen, Yi Jen; Chen, Yao Chang; Chan, Paul; Lin, Cheng I.; Chen, Shih Ann.

In: Journal of Biomedical Science, Vol. 10, No. 5, 2003, p. 535-543.

Research output: Contribution to journalArticle

Chen, Yi Jen ; Chen, Yao Chang ; Chan, Paul ; Lin, Cheng I. ; Chen, Shih Ann. / Temperature regulates the arrhythmogenic activity of pulmonary vein cardiomyocytes. In: Journal of Biomedical Science. 2003 ; Vol. 10, No. 5. pp. 535-543.
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