Technical aspects of the piezo, laser-assisted, and conventional methods for nuclear transfer of mouse oocytes and their efficiency and efficacy

Piezo minimizes damage of the ooplasmic membrane at injection

Shee Uan Chen, Kuang Han Chao, Chia Y.I. Chang, Fon Jou Hsieh, Hong Nerng Ho, Yu Shih Yang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Assessment of the advantages and disadvantages of the piezo, laser, and conventional methods for nuclear transfer has remained elusive. Furthermore, although the piezo method had been used by some investigators for research of sperm injection and nuclear transfer for several years, many researchers have failed to operate the technique smoothly and achieve reproducible results. The procedures of nuclear transfer using piezo were ascertained and described in detail. Mouse oocytes were enucleated, and injected with cumulus cells using the piezo, laser, or conventional methods. We investigated the time needed and survival of nuclear transfer. Development was compared among the three methods and parthenogenetic control specimens. The average time of nuclear transfer for each oocyte was significantly shorter using the piezo (118 ± 9 s) and laser methods (120 ± 11 s) than using the conventional method (170 ± 11 s). The damage rate was smaller for the piezo group (10%) than the laser (37%) and conventional (40%) groups. The percentages of blastocyst formation (14%, 12%, and 11%) and the number of nuclei of blastocysts (54 ± 13, 51 ± 11, and 52 ± 12) were similar among the piezo, laser, and conventional groups, but significantly lower than for the control group (83%, 105 ± 14). The piezo technique is more efficient than the conventional method for nuclear transfer, The laser method is easy to operate, but the equipment is expensive. In addition, piezo induced fewer traumas while breaking the membrane than the aspiration techniques used in the laser and conventional methods.

Original languageEnglish
Pages (from-to)344-351
Number of pages8
JournalJournal of Experimental Zoology Part A: Comparative Experimental Biology
Volume301
Issue number4
DOIs
Publication statusPublished - Apr 1 2004
Externally publishedYes

Fingerprint

Oocytes
Lasers
Injections
Membranes
Blastocyst
Research Personnel
Cumulus Cells
Spermatozoa
Equipment and Supplies
Control Groups
Wounds and Injuries
Research

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

@article{6bdda319c94545d1bf9748bccf94bc59,
title = "Technical aspects of the piezo, laser-assisted, and conventional methods for nuclear transfer of mouse oocytes and their efficiency and efficacy: Piezo minimizes damage of the ooplasmic membrane at injection",
abstract = "Assessment of the advantages and disadvantages of the piezo, laser, and conventional methods for nuclear transfer has remained elusive. Furthermore, although the piezo method had been used by some investigators for research of sperm injection and nuclear transfer for several years, many researchers have failed to operate the technique smoothly and achieve reproducible results. The procedures of nuclear transfer using piezo were ascertained and described in detail. Mouse oocytes were enucleated, and injected with cumulus cells using the piezo, laser, or conventional methods. We investigated the time needed and survival of nuclear transfer. Development was compared among the three methods and parthenogenetic control specimens. The average time of nuclear transfer for each oocyte was significantly shorter using the piezo (118 ± 9 s) and laser methods (120 ± 11 s) than using the conventional method (170 ± 11 s). The damage rate was smaller for the piezo group (10{\%}) than the laser (37{\%}) and conventional (40{\%}) groups. The percentages of blastocyst formation (14{\%}, 12{\%}, and 11{\%}) and the number of nuclei of blastocysts (54 ± 13, 51 ± 11, and 52 ± 12) were similar among the piezo, laser, and conventional groups, but significantly lower than for the control group (83{\%}, 105 ± 14). The piezo technique is more efficient than the conventional method for nuclear transfer, The laser method is easy to operate, but the equipment is expensive. In addition, piezo induced fewer traumas while breaking the membrane than the aspiration techniques used in the laser and conventional methods.",
author = "Chen, {Shee Uan} and Chao, {Kuang Han} and Chang, {Chia Y.I.} and Hsieh, {Fon Jou} and Ho, {Hong Nerng} and Yang, {Yu Shih}",
year = "2004",
month = "4",
day = "1",
doi = "10.1002/jez.a.20037",
language = "English",
volume = "301",
pages = "344--351",
journal = "Journal of Experimental Zoology Part A: Comparative Experimental Biology",
issn = "1548-8969",
publisher = "John Wiley and Sons Inc.",
number = "4",

}

TY - JOUR

T1 - Technical aspects of the piezo, laser-assisted, and conventional methods for nuclear transfer of mouse oocytes and their efficiency and efficacy

T2 - Piezo minimizes damage of the ooplasmic membrane at injection

AU - Chen, Shee Uan

AU - Chao, Kuang Han

AU - Chang, Chia Y.I.

AU - Hsieh, Fon Jou

AU - Ho, Hong Nerng

AU - Yang, Yu Shih

PY - 2004/4/1

Y1 - 2004/4/1

N2 - Assessment of the advantages and disadvantages of the piezo, laser, and conventional methods for nuclear transfer has remained elusive. Furthermore, although the piezo method had been used by some investigators for research of sperm injection and nuclear transfer for several years, many researchers have failed to operate the technique smoothly and achieve reproducible results. The procedures of nuclear transfer using piezo were ascertained and described in detail. Mouse oocytes were enucleated, and injected with cumulus cells using the piezo, laser, or conventional methods. We investigated the time needed and survival of nuclear transfer. Development was compared among the three methods and parthenogenetic control specimens. The average time of nuclear transfer for each oocyte was significantly shorter using the piezo (118 ± 9 s) and laser methods (120 ± 11 s) than using the conventional method (170 ± 11 s). The damage rate was smaller for the piezo group (10%) than the laser (37%) and conventional (40%) groups. The percentages of blastocyst formation (14%, 12%, and 11%) and the number of nuclei of blastocysts (54 ± 13, 51 ± 11, and 52 ± 12) were similar among the piezo, laser, and conventional groups, but significantly lower than for the control group (83%, 105 ± 14). The piezo technique is more efficient than the conventional method for nuclear transfer, The laser method is easy to operate, but the equipment is expensive. In addition, piezo induced fewer traumas while breaking the membrane than the aspiration techniques used in the laser and conventional methods.

AB - Assessment of the advantages and disadvantages of the piezo, laser, and conventional methods for nuclear transfer has remained elusive. Furthermore, although the piezo method had been used by some investigators for research of sperm injection and nuclear transfer for several years, many researchers have failed to operate the technique smoothly and achieve reproducible results. The procedures of nuclear transfer using piezo were ascertained and described in detail. Mouse oocytes were enucleated, and injected with cumulus cells using the piezo, laser, or conventional methods. We investigated the time needed and survival of nuclear transfer. Development was compared among the three methods and parthenogenetic control specimens. The average time of nuclear transfer for each oocyte was significantly shorter using the piezo (118 ± 9 s) and laser methods (120 ± 11 s) than using the conventional method (170 ± 11 s). The damage rate was smaller for the piezo group (10%) than the laser (37%) and conventional (40%) groups. The percentages of blastocyst formation (14%, 12%, and 11%) and the number of nuclei of blastocysts (54 ± 13, 51 ± 11, and 52 ± 12) were similar among the piezo, laser, and conventional groups, but significantly lower than for the control group (83%, 105 ± 14). The piezo technique is more efficient than the conventional method for nuclear transfer, The laser method is easy to operate, but the equipment is expensive. In addition, piezo induced fewer traumas while breaking the membrane than the aspiration techniques used in the laser and conventional methods.

UR - http://www.scopus.com/inward/record.url?scp=1942488984&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1942488984&partnerID=8YFLogxK

U2 - 10.1002/jez.a.20037

DO - 10.1002/jez.a.20037

M3 - Article

VL - 301

SP - 344

EP - 351

JO - Journal of Experimental Zoology Part A: Comparative Experimental Biology

JF - Journal of Experimental Zoology Part A: Comparative Experimental Biology

SN - 1548-8969

IS - 4

ER -