Tc17 CD8 T cells

Functional plasticity and subset diversity

Hung Rong Yen, Timothy J. Harris, Satoshi Wada, Joseph F. Grosso, Derese Getnet, Monica V. Goldberg, Kai Li Liang, Tullia C. Bruno, Kristin J. Pyle, Siaw Li Chan, Robert A. Anders, Cornelia L. Trimble, Adam J. Adler, Tzou Yien Lin, Drew M. Pardoll, Ching Tai Huang, Charles G. Drake

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

IL-17-secreting CD8 T cells (Tc17) have been described in several settings, but little is known regarding their functional characteristics. While Tc1 cells produced IFN-γ and efficiently killed targets, Tc17 cells lacked lytic function in vitro. Interestingly, the small numbers of IFN-γ-positive or IL-17/IFN-γ-double-positive cells generated under Tc17 conditions also lacked lytic activity and expressed a similar pattern of cell surface proteins to IL-17-producing cells. As is the case for Th17 (CD4) cells, STAT3 is important for Tc17 polarization, both in vitro and in vivo. Adoptive transfer of highly purified, Ag-specific IL-17-secreting Tc17 cells into Ag-bearing hosts resulted in near complete conversion to an IFN-γ-secreting phenotype and substantial pulmonary pathology, demonstrating functional plasticity. Tc17 also accumulated to a greater extent than did Tc1 cells, suggesting that adoptive transfer of CD8 T cells cultured in Tc17 conditions may have therapeutic potential for diseases in which IFN-γ-producing cells are desired.

Original languageEnglish
Pages (from-to)7161-7168
Number of pages8
JournalJournal of Immunology
Volume183
Issue number11
DOIs
Publication statusPublished - Dec 1 2009
Externally publishedYes

Fingerprint

T-Lymphocyte Subsets
Interleukin-17
Adoptive Transfer
T-Lymphocytes
Th17 Cells
Cell Plasticity
Membrane Proteins
Pathology
Phenotype
Lung

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Yen, H. R., Harris, T. J., Wada, S., Grosso, J. F., Getnet, D., Goldberg, M. V., ... Drake, C. G. (2009). Tc17 CD8 T cells: Functional plasticity and subset diversity. Journal of Immunology, 183(11), 7161-7168. https://doi.org/10.4049/jimmunol.0900368

Tc17 CD8 T cells : Functional plasticity and subset diversity. / Yen, Hung Rong; Harris, Timothy J.; Wada, Satoshi; Grosso, Joseph F.; Getnet, Derese; Goldberg, Monica V.; Liang, Kai Li; Bruno, Tullia C.; Pyle, Kristin J.; Chan, Siaw Li; Anders, Robert A.; Trimble, Cornelia L.; Adler, Adam J.; Lin, Tzou Yien; Pardoll, Drew M.; Huang, Ching Tai; Drake, Charles G.

In: Journal of Immunology, Vol. 183, No. 11, 01.12.2009, p. 7161-7168.

Research output: Contribution to journalArticle

Yen, HR, Harris, TJ, Wada, S, Grosso, JF, Getnet, D, Goldberg, MV, Liang, KL, Bruno, TC, Pyle, KJ, Chan, SL, Anders, RA, Trimble, CL, Adler, AJ, Lin, TY, Pardoll, DM, Huang, CT & Drake, CG 2009, 'Tc17 CD8 T cells: Functional plasticity and subset diversity', Journal of Immunology, vol. 183, no. 11, pp. 7161-7168. https://doi.org/10.4049/jimmunol.0900368
Yen HR, Harris TJ, Wada S, Grosso JF, Getnet D, Goldberg MV et al. Tc17 CD8 T cells: Functional plasticity and subset diversity. Journal of Immunology. 2009 Dec 1;183(11):7161-7168. https://doi.org/10.4049/jimmunol.0900368
Yen, Hung Rong ; Harris, Timothy J. ; Wada, Satoshi ; Grosso, Joseph F. ; Getnet, Derese ; Goldberg, Monica V. ; Liang, Kai Li ; Bruno, Tullia C. ; Pyle, Kristin J. ; Chan, Siaw Li ; Anders, Robert A. ; Trimble, Cornelia L. ; Adler, Adam J. ; Lin, Tzou Yien ; Pardoll, Drew M. ; Huang, Ching Tai ; Drake, Charles G. / Tc17 CD8 T cells : Functional plasticity and subset diversity. In: Journal of Immunology. 2009 ; Vol. 183, No. 11. pp. 7161-7168.
@article{bd44ba09c0934d1f9d8fee4eaff48f3e,
title = "Tc17 CD8 T cells: Functional plasticity and subset diversity",
abstract = "IL-17-secreting CD8 T cells (Tc17) have been described in several settings, but little is known regarding their functional characteristics. While Tc1 cells produced IFN-γ and efficiently killed targets, Tc17 cells lacked lytic function in vitro. Interestingly, the small numbers of IFN-γ-positive or IL-17/IFN-γ-double-positive cells generated under Tc17 conditions also lacked lytic activity and expressed a similar pattern of cell surface proteins to IL-17-producing cells. As is the case for Th17 (CD4) cells, STAT3 is important for Tc17 polarization, both in vitro and in vivo. Adoptive transfer of highly purified, Ag-specific IL-17-secreting Tc17 cells into Ag-bearing hosts resulted in near complete conversion to an IFN-γ-secreting phenotype and substantial pulmonary pathology, demonstrating functional plasticity. Tc17 also accumulated to a greater extent than did Tc1 cells, suggesting that adoptive transfer of CD8 T cells cultured in Tc17 conditions may have therapeutic potential for diseases in which IFN-γ-producing cells are desired.",
author = "Yen, {Hung Rong} and Harris, {Timothy J.} and Satoshi Wada and Grosso, {Joseph F.} and Derese Getnet and Goldberg, {Monica V.} and Liang, {Kai Li} and Bruno, {Tullia C.} and Pyle, {Kristin J.} and Chan, {Siaw Li} and Anders, {Robert A.} and Trimble, {Cornelia L.} and Adler, {Adam J.} and Lin, {Tzou Yien} and Pardoll, {Drew M.} and Huang, {Ching Tai} and Drake, {Charles G.}",
year = "2009",
month = "12",
day = "1",
doi = "10.4049/jimmunol.0900368",
language = "English",
volume = "183",
pages = "7161--7168",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "11",

}

TY - JOUR

T1 - Tc17 CD8 T cells

T2 - Functional plasticity and subset diversity

AU - Yen, Hung Rong

AU - Harris, Timothy J.

AU - Wada, Satoshi

AU - Grosso, Joseph F.

AU - Getnet, Derese

AU - Goldberg, Monica V.

AU - Liang, Kai Li

AU - Bruno, Tullia C.

AU - Pyle, Kristin J.

AU - Chan, Siaw Li

AU - Anders, Robert A.

AU - Trimble, Cornelia L.

AU - Adler, Adam J.

AU - Lin, Tzou Yien

AU - Pardoll, Drew M.

AU - Huang, Ching Tai

AU - Drake, Charles G.

PY - 2009/12/1

Y1 - 2009/12/1

N2 - IL-17-secreting CD8 T cells (Tc17) have been described in several settings, but little is known regarding their functional characteristics. While Tc1 cells produced IFN-γ and efficiently killed targets, Tc17 cells lacked lytic function in vitro. Interestingly, the small numbers of IFN-γ-positive or IL-17/IFN-γ-double-positive cells generated under Tc17 conditions also lacked lytic activity and expressed a similar pattern of cell surface proteins to IL-17-producing cells. As is the case for Th17 (CD4) cells, STAT3 is important for Tc17 polarization, both in vitro and in vivo. Adoptive transfer of highly purified, Ag-specific IL-17-secreting Tc17 cells into Ag-bearing hosts resulted in near complete conversion to an IFN-γ-secreting phenotype and substantial pulmonary pathology, demonstrating functional plasticity. Tc17 also accumulated to a greater extent than did Tc1 cells, suggesting that adoptive transfer of CD8 T cells cultured in Tc17 conditions may have therapeutic potential for diseases in which IFN-γ-producing cells are desired.

AB - IL-17-secreting CD8 T cells (Tc17) have been described in several settings, but little is known regarding their functional characteristics. While Tc1 cells produced IFN-γ and efficiently killed targets, Tc17 cells lacked lytic function in vitro. Interestingly, the small numbers of IFN-γ-positive or IL-17/IFN-γ-double-positive cells generated under Tc17 conditions also lacked lytic activity and expressed a similar pattern of cell surface proteins to IL-17-producing cells. As is the case for Th17 (CD4) cells, STAT3 is important for Tc17 polarization, both in vitro and in vivo. Adoptive transfer of highly purified, Ag-specific IL-17-secreting Tc17 cells into Ag-bearing hosts resulted in near complete conversion to an IFN-γ-secreting phenotype and substantial pulmonary pathology, demonstrating functional plasticity. Tc17 also accumulated to a greater extent than did Tc1 cells, suggesting that adoptive transfer of CD8 T cells cultured in Tc17 conditions may have therapeutic potential for diseases in which IFN-γ-producing cells are desired.

UR - http://www.scopus.com/inward/record.url?scp=73349101421&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=73349101421&partnerID=8YFLogxK

U2 - 10.4049/jimmunol.0900368

DO - 10.4049/jimmunol.0900368

M3 - Article

VL - 183

SP - 7161

EP - 7168

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 11

ER -