Taurine exerts robust protection against hypoxia and oxygen/glucose deprivation in human neuroblastoma cell culture

Po Chih Chen, Chunliu Pan, Payam M. Gharibani, Howard Prentice, Jang Yen Wu

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Stroke is one of the leading causes of mortality and disability worldwide. There is no effective treatment for stroke despite extensive research. Taurine is a free amino acid which is present at high concentrations in a range of organs including the brain, heart, and retina in mammalian systems. It had been shown that taurine can significantly increase cell survival under stroke conditions using both in vivo and in vitro models. Recently, we have found that several agents including granulocyte colony-stimulating factor (G-CSF), a stem cell enhancer and facilitator; S -methyl- N -diethylthiolcarbamate sulfoxide (DETC-MeSO), an NMDA receptor partial antagonist; sulindac, a potent antioxidant; and taurine, a neuroprotectant and calcium regulator, are effective in protecting against stroke-induced neuronal injury when used alone or in combination in both animal and tissue/cell culture models. In this chapter, we demonstrate that taurine can protect human neuroblastoma cells measured by ATP assay under conditions of hypoxia or oxygen/glucose deprivation (OGD). In addition, we found that taurine exerts its protective function by suppressing the OGD-induced upregulation of endoplasmic reticulum (ER) stress markers and proapoptotic proteins. A model depicting the mode of action of taurine in protecting neuroblastoma cells under OGD conditions is presented.

Original languageEnglish
Title of host publicationTaurine 8
Subtitle of host publicationVolume 1: The Nervous System, Immune System, Diabetes and the Cardiovascular System
PublisherSpringer New York LLC
Pages167-175
Number of pages9
ISBN (Print)9781461461296
DOIs
Publication statusPublished - 2013

Publication series

NameAdvances in Experimental Medicine and Biology
Volume775
ISSN (Print)0065-2598

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Chen, P. C., Pan, C., Gharibani, P. M., Prentice, H., & Wu, J. Y. (2013). Taurine exerts robust protection against hypoxia and oxygen/glucose deprivation in human neuroblastoma cell culture. In Taurine 8: Volume 1: The Nervous System, Immune System, Diabetes and the Cardiovascular System (pp. 167-175). (Advances in Experimental Medicine and Biology; Vol. 775). Springer New York LLC. https://doi.org/10.1007/978-1-4614-6130-2_14