Targeting cancer stem cells in breast cancer: Potential anticancer properties of 6-shogaol and pterostilbene

Chi Hao Wu, Bo Han Hong, Chi Tang Ho, Gow Chin Yen

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Breast cancer stem cells (BCSCs) constitute a small fraction of the primary tumor that can self-renew and become a drug-resistant cell population, thus limiting the treatment effects of chemotherapeutic drugs. The present study evaluated the cytotoxic effects of five phytochemicals including 6-gingerol (6-G), 6-shogaol (6-S), 5-hydroxy-3,6,7,8,3′,4′-hexamethoxyflavone (5-HF), nobiletin (NOL), and pterostilbene (PTE) on MCF-7 breast cancer cells and BCSCs. The results showed that 6-G, 6-S, and PTE selectively killed BCSCs and had high sensitivity for BCSCs isolated from MCF-7 cells that expressed the surface antigen CD44+/CD24-. 6-S and PTE induced cell necrosis phenomena such as membrane injury and bleb formation in BCSCs and inhibited mammosphere formation. In addition, 6-S and PTE increased the sensitivity of isolated BCSCs to chemotherapeutic drugs and significantly increased the anticancer activity of paclitaxel. Analysis of the underlying mechanism showed that 6-S and PTE decreased the expression of the surface antigen CD44 on BCSCs and promoted β-catenin phosphorylation through the inhibition of hedgehog/Akt/GSK3β signaling, thus decreasing the protein expression of downstream c-Myc and cyclin D1 and reducing BCSC stemness.

Original languageEnglish
Pages (from-to)2432-2441
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number9
DOIs
Publication statusPublished - 2015

Keywords

  • 6-shogaol
  • breast cancer stem cells
  • hedgehog
  • mammospheres
  • pterostilbene

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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