Targeting cancer stem cells in breast cancer

Potential anticancer properties of 6-shogaol and pterostilbene

Chi Hao Wu, Bo Han Hong, Chi Tang Ho, Gow Chin Yen

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Breast cancer stem cells (BCSCs) constitute a small fraction of the primary tumor that can self-renew and become a drug-resistant cell population, thus limiting the treatment effects of chemotherapeutic drugs. The present study evaluated the cytotoxic effects of five phytochemicals including 6-gingerol (6-G), 6-shogaol (6-S), 5-hydroxy-3,6,7,8,3′,4′-hexamethoxyflavone (5-HF), nobiletin (NOL), and pterostilbene (PTE) on MCF-7 breast cancer cells and BCSCs. The results showed that 6-G, 6-S, and PTE selectively killed BCSCs and had high sensitivity for BCSCs isolated from MCF-7 cells that expressed the surface antigen CD44+/CD24-. 6-S and PTE induced cell necrosis phenomena such as membrane injury and bleb formation in BCSCs and inhibited mammosphere formation. In addition, 6-S and PTE increased the sensitivity of isolated BCSCs to chemotherapeutic drugs and significantly increased the anticancer activity of paclitaxel. Analysis of the underlying mechanism showed that 6-S and PTE decreased the expression of the surface antigen CD44 on BCSCs and promoted β-catenin phosphorylation through the inhibition of hedgehog/Akt/GSK3β signaling, thus decreasing the protein expression of downstream c-Myc and cyclin D1 and reducing BCSC stemness.

Original languageEnglish
Pages (from-to)2432-2441
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number9
DOIs
Publication statusPublished - 2015

Fingerprint

Neoplastic Stem Cells
Stem cells
breast neoplasms
stem cells
Breast Neoplasms
Surface Antigens
surface antigens
drugs
Cells
Pharmaceutical Preparations
Catenins
Phosphorylation
neoplasm cells
pterostilbene
shogaol
Cyclin D1
Phytochemicals
paclitaxel
Erinaceidae
Paclitaxel

Keywords

  • 6-shogaol
  • breast cancer stem cells
  • hedgehog
  • mammospheres
  • pterostilbene

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Targeting cancer stem cells in breast cancer : Potential anticancer properties of 6-shogaol and pterostilbene. / Wu, Chi Hao; Hong, Bo Han; Ho, Chi Tang; Yen, Gow Chin.

In: Journal of Agricultural and Food Chemistry, Vol. 63, No. 9, 2015, p. 2432-2441.

Research output: Contribution to journalArticle

Wu, Chi Hao ; Hong, Bo Han ; Ho, Chi Tang ; Yen, Gow Chin. / Targeting cancer stem cells in breast cancer : Potential anticancer properties of 6-shogaol and pterostilbene. In: Journal of Agricultural and Food Chemistry. 2015 ; Vol. 63, No. 9. pp. 2432-2441.
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