Targeted methylation of two tumor suppressor genes is sufficient to transform mesenchymal stem cells into cancer stem/initiating cells

I. Wen Teng, Pei Chi Hou, Kuan Der Lee, Pei Yi Chu, Kun Tu Yeh, Victor X. Jin, Min Jen Tseng, Shaw Jenq Tsai, Yu Sun Chang, Chi Sheng Wu, H. Sunny Sun, Kuen Daw Tsai, Long Bin Jeng, Kenneth P. Nephew, Tim H.M. Huang, Shu Huei Hsiao, Yu Wei Leu

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Although DNA hypermethylation within promoter CpG islands is highly correlated with tumorigenesis, it has not been established whether DNA hypermethylation within a specific tumor suppressor gene (TSG) is sufficient to fully transform a somatic stem cell. In this study, we addressed this question using a novel targeted DNA methylation technique to methylate the promoters of HIC1 and RassF1A, two well-established TSGs, along with a two-component reporter system to visualize successful targeting of human bone marrow-derived mesenchymal stem cells (MSC) as a model cell system. MSCs harboring targeted promoter methylations of HIC1/RassF1A displayed several features of cancer stem/initiating cells including loss of anchorage dependence, increased colony formation capability, drug resistance, and pluripotency. Notably, inoculation of immunodeficient mice with low numbers of targeted MSC resulted in tumor formation, and subsequent serial xenotransplantation and immunohistochemistry confirmed the presence of stem cell markers and MSC lineage in tumor xenografts. Consistent with the expected mechanism of TSG hypermethylation, treatment of the targeted MSC with a DNA methyltransferase inhibitor reversed their tumorigenic phenotype. To our knowledge, this is the first direct demonstration that aberrant TSG hypermethylation is sufficient to transform a somatic stem cell into a fully malignant cell with cancer stem/initiating properties.

Original languageEnglish
Pages (from-to)4653-4663
Number of pages11
JournalCancer Research
Volume71
Issue number13
DOIs
Publication statusPublished - Jul 1 2011
Externally publishedYes

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Neoplastic Stem Cells
Tumor Suppressor Genes
Mesenchymal Stromal Cells
Methylation
Adult Stem Cells
DNA
Heterologous Transplantation
CpG Islands
Methyltransferases
Cell Lineage
DNA Methylation
Drug Resistance
Heterografts
Neoplasms
Carcinogenesis
Stem Cells
Bone Marrow
Immunohistochemistry
Phenotype
Therapeutics

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Targeted methylation of two tumor suppressor genes is sufficient to transform mesenchymal stem cells into cancer stem/initiating cells. / Teng, I. Wen; Hou, Pei Chi; Lee, Kuan Der; Chu, Pei Yi; Yeh, Kun Tu; Jin, Victor X.; Tseng, Min Jen; Tsai, Shaw Jenq; Chang, Yu Sun; Wu, Chi Sheng; Sun, H. Sunny; Tsai, Kuen Daw; Jeng, Long Bin; Nephew, Kenneth P.; Huang, Tim H.M.; Hsiao, Shu Huei; Leu, Yu Wei.

In: Cancer Research, Vol. 71, No. 13, 01.07.2011, p. 4653-4663.

Research output: Contribution to journalArticle

Teng, IW, Hou, PC, Lee, KD, Chu, PY, Yeh, KT, Jin, VX, Tseng, MJ, Tsai, SJ, Chang, YS, Wu, CS, Sun, HS, Tsai, KD, Jeng, LB, Nephew, KP, Huang, THM, Hsiao, SH & Leu, YW 2011, 'Targeted methylation of two tumor suppressor genes is sufficient to transform mesenchymal stem cells into cancer stem/initiating cells', Cancer Research, vol. 71, no. 13, pp. 4653-4663. https://doi.org/10.1158/0008-5472.CAN-10-3418
Teng, I. Wen ; Hou, Pei Chi ; Lee, Kuan Der ; Chu, Pei Yi ; Yeh, Kun Tu ; Jin, Victor X. ; Tseng, Min Jen ; Tsai, Shaw Jenq ; Chang, Yu Sun ; Wu, Chi Sheng ; Sun, H. Sunny ; Tsai, Kuen Daw ; Jeng, Long Bin ; Nephew, Kenneth P. ; Huang, Tim H.M. ; Hsiao, Shu Huei ; Leu, Yu Wei. / Targeted methylation of two tumor suppressor genes is sufficient to transform mesenchymal stem cells into cancer stem/initiating cells. In: Cancer Research. 2011 ; Vol. 71, No. 13. pp. 4653-4663.
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