Abstract
Chemotherapy damages the reproductive system by enhancing apoptosis, and evidence suggests that targeted anti-apoptotic therapy may preserve fertility in patients receiving chemotherapy. To investigate the protective effect of sphingosine-1-phosphate (S1P) on chemotherapeutic agent-induced ovarian gonadotoxicity, busulfan-treated female mice were pre-treated with low (0.5 mM) and high (2.0 mM) doses of S1P or vehicle 1 h before busulfan injection. In the S1P groups, each mouse was injected with low-dose S1P in one ovary and high-dose S1P in the contralateral ovary. Four weeks later, the ovaries were removed for histological and biochemical examinations. Caspase 3 immunoreactivity was greater in mice treated with busulfan compared with mice pre-treated with S1P, in which more primordial follicles were observed (P <0.05). The mRNA level of anti-Müllerian hormone was higher in mice pre-treated with S1P than those that received busulfan only, indicating a better ovarian function in mice pre-treated with S1P. No difference was observed in the levels of growth differentiation factor-9 among all groups. In conclusion, S1P protects primordial follicles from chemotherapy-induced gonadotoxicity, and may partially preserve ovarian function.
| Original language | English |
|---|---|
| Pages (from-to) | 612-620 |
| Number of pages | 9 |
| Journal | Reproductive BioMedicine Online |
| Volume | 29 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Nov 1 2014 |
Fingerprint
Keywords
- anti-apoptotic therapy
- anti-Müllerian hormone
- caspase-3
- fertility preservation
- gonadotoxicity
- sphingosine-1-phosphate
ASJC Scopus subject areas
- Reproductive Medicine
- Developmental Biology
- Medicine(all)
Cite this
Targeted anti-apoptosis activity for ovarian protection against chemotherapy-induced ovarian gonadotoxicity. / Tan, Shun Jen; Lee, Li Jen; Tzeng, Chii Ruey; Wang, Chia-Woei; Hsu, Ming I.; Chen, Chi Huang.
In: Reproductive BioMedicine Online, Vol. 29, No. 5, 01.11.2014, p. 612-620.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Targeted anti-apoptosis activity for ovarian protection against chemotherapy-induced ovarian gonadotoxicity
AU - Tan, Shun Jen
AU - Lee, Li Jen
AU - Tzeng, Chii Ruey
AU - Wang, Chia-Woei
AU - Hsu, Ming I.
AU - Chen, Chi Huang
PY - 2014/11/1
Y1 - 2014/11/1
N2 - Chemotherapy damages the reproductive system by enhancing apoptosis, and evidence suggests that targeted anti-apoptotic therapy may preserve fertility in patients receiving chemotherapy. To investigate the protective effect of sphingosine-1-phosphate (S1P) on chemotherapeutic agent-induced ovarian gonadotoxicity, busulfan-treated female mice were pre-treated with low (0.5 mM) and high (2.0 mM) doses of S1P or vehicle 1 h before busulfan injection. In the S1P groups, each mouse was injected with low-dose S1P in one ovary and high-dose S1P in the contralateral ovary. Four weeks later, the ovaries were removed for histological and biochemical examinations. Caspase 3 immunoreactivity was greater in mice treated with busulfan compared with mice pre-treated with S1P, in which more primordial follicles were observed (P <0.05). The mRNA level of anti-Müllerian hormone was higher in mice pre-treated with S1P than those that received busulfan only, indicating a better ovarian function in mice pre-treated with S1P. No difference was observed in the levels of growth differentiation factor-9 among all groups. In conclusion, S1P protects primordial follicles from chemotherapy-induced gonadotoxicity, and may partially preserve ovarian function.
AB - Chemotherapy damages the reproductive system by enhancing apoptosis, and evidence suggests that targeted anti-apoptotic therapy may preserve fertility in patients receiving chemotherapy. To investigate the protective effect of sphingosine-1-phosphate (S1P) on chemotherapeutic agent-induced ovarian gonadotoxicity, busulfan-treated female mice were pre-treated with low (0.5 mM) and high (2.0 mM) doses of S1P or vehicle 1 h before busulfan injection. In the S1P groups, each mouse was injected with low-dose S1P in one ovary and high-dose S1P in the contralateral ovary. Four weeks later, the ovaries were removed for histological and biochemical examinations. Caspase 3 immunoreactivity was greater in mice treated with busulfan compared with mice pre-treated with S1P, in which more primordial follicles were observed (P <0.05). The mRNA level of anti-Müllerian hormone was higher in mice pre-treated with S1P than those that received busulfan only, indicating a better ovarian function in mice pre-treated with S1P. No difference was observed in the levels of growth differentiation factor-9 among all groups. In conclusion, S1P protects primordial follicles from chemotherapy-induced gonadotoxicity, and may partially preserve ovarian function.
KW - anti-apoptotic therapy
KW - anti-Müllerian hormone
KW - caspase-3
KW - fertility preservation
KW - gonadotoxicity
KW - sphingosine-1-phosphate
UR - http://www.scopus.com/inward/record.url?scp=84908395184&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84908395184&partnerID=8YFLogxK
U2 - 10.1016/j.rbmo.2014.07.014
DO - 10.1016/j.rbmo.2014.07.014
M3 - Article
C2 - 25246113
AN - SCOPUS:84908395184
VL - 29
SP - 612
EP - 620
JO - Reproductive BioMedicine Online
JF - Reproductive BioMedicine Online
SN - 1472-6483
IS - 5
ER -