Tanshinone IIA prevents doxorubicin-induced cardiomyocyte apoptosis through Akt-dependent pathway

Hong Jye Hong, Ju Chi Liu, Po Yuan Chen, Jin Jer Chen, Paul Chan, Tzu-Hurng Cheng

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Background: Doxorubicin, one of the original anthracyclines, remains among the most effective anticancer drugs ever developed. Clinical use of doxorubicin is, however, greatly limited by its serious adverse cardiac effects that may ultimately lead to cardiomyopathy and heart failure. Tanshinone IIA is the main effective component of Salvia miltiorrhiza known as 'Danshen' in traditional Chinese medicine for treating cardiovascular disorders. The objective of this study was set to evaluate the protective effect of tanshinone IIA on doxorubicin-induced cardiomyocyte apoptosis, and to explore its intracellular mechanism(s). Methods: Primary cultured neonatal rat cardiomyocytes were treated with the vehicle, doxorubicin (1 μM), tanshinone IIA (0.1, 0.3, 1 and 3 μM), or tanshinone IIA plus doxorubicin. Results: We found that tanshinone IIA (1 and 3 μM) inhibited doxorubicin-induced reactive oxygen species generation, reduced the quantity of cleaved caspase-3 and cytosol cytochrome c, and increased BcL-xL expression, resulting in protecting cardiomyocytes from doxorubicin-induced apoptosis. In addition, Akt phosphorylation was enhanced by tanshinone IIA treatment in cardiomyocytes. The wortmannin (100 nM), LY294002 (10 nM), and siRNA transfection for Akt significantly reduced tanshinone IIA-induced protective effect. Conclusions: These findings suggest that tanshinone IIA protects cardiomyocytes from doxorubicin-induced apoptosis in part through Akt-signaling pathways, which may potentially protect the heart from the severe toxicity of doxorubicin.

Original languageEnglish
Pages (from-to)174-179
Number of pages6
JournalInternational Journal of Cardiology
Volume157
Issue number2
DOIs
Publication statusPublished - May 31 2012

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Cardiac Myocytes
Doxorubicin
Apoptosis
Salvia miltiorrhiza
tanshinone
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Anthracyclines
Chinese Traditional Medicine
Cytochromes c
Cardiomyopathies
Caspase 3
Cytosol
Small Interfering RNA
Transfection
Reactive Oxygen Species
Heart Failure
Phosphorylation
Pharmaceutical Preparations

Keywords

  • Akt
  • Cardiomyocyte apoptosis
  • Doxorubicin
  • Tanshinone IIA
  • Traditional Chinese medicine

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Tanshinone IIA prevents doxorubicin-induced cardiomyocyte apoptosis through Akt-dependent pathway. / Hong, Hong Jye; Liu, Ju Chi; Chen, Po Yuan; Chen, Jin Jer; Chan, Paul; Cheng, Tzu-Hurng.

In: International Journal of Cardiology, Vol. 157, No. 2, 31.05.2012, p. 174-179.

Research output: Contribution to journalArticle

Hong, Hong Jye ; Liu, Ju Chi ; Chen, Po Yuan ; Chen, Jin Jer ; Chan, Paul ; Cheng, Tzu-Hurng. / Tanshinone IIA prevents doxorubicin-induced cardiomyocyte apoptosis through Akt-dependent pathway. In: International Journal of Cardiology. 2012 ; Vol. 157, No. 2. pp. 174-179.
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