T-type calcium current in electrical activity of cardiomyocytes isolated from rabbit pulmonary vein

Yao Chang Chen, Shih Ann Chen, Yi Jen Chen, Ching Tai Tai, Paul Chan, Cheng I. Lin

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Introduction: Pulmonary veins (PVs) are known to initiate paroxysmal atrial fibrillation. T-type calcium current (ICa-T) has a role in normal and abnormal automaticity of cardiomyocytes. The aim of this study was to evaluate whether ICa-T contributes to PV electrical activity. Methods and Results: By whole-cell clamp techniques in rabbit myocytes, I Ca-T was identified in 12 (39%) of 31 PV cardiomyocytes with pacemaker activity, 2 (9%) of 23 PV cardiomyocytes without pacemaker activity, and 2 (15%) of 13 atrial myocytes (P <0.05). Maximum ICa-L and ICa-T densities from PV cardiomyocytes with pacemaker activity were 6.87 ± 2.17 pA/pF and 1.38 ± 0.69 pA/pF, respectively. Nickel (40 μM) decreased the spontaneous activity in 5 (36%) of 14 PV cardiomyocytes (3.1 ± 0.6 Hz vs 2.2 ± 0.5 Hz, P <0.05), reduced the amplitudes of delayed afterdepolarization from 13 ± 1 mV to 7 ± 1 mV (n = 4, P <0.05) and inhibited transient inward currents from 1.2 ± 0.2 pA/pF to 0.7 ± 0.1 pA/pF (n = 11, P <0.01). Conclusions: We conclude that ICa-T contributes to PV pacemaker activity and triggered activity, which are of functional importance in PV arrhythmogenesis.

Original languageEnglish
Pages (from-to)567-571
Number of pages5
JournalJournal of Cardiovascular Electrophysiology
Volume15
Issue number5
Publication statusPublished - May 2004

Fingerprint

Pulmonary Veins
Cardiac Myocytes
Rabbits
Calcium
Muscle Cells
Nickel
Atrial Fibrillation

Keywords

  • Calcium channel
  • Nickel
  • Pulmonary vein
  • Transient inward current

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology

Cite this

T-type calcium current in electrical activity of cardiomyocytes isolated from rabbit pulmonary vein. / Chen, Yao Chang; Chen, Shih Ann; Chen, Yi Jen; Tai, Ching Tai; Chan, Paul; Lin, Cheng I.

In: Journal of Cardiovascular Electrophysiology, Vol. 15, No. 5, 05.2004, p. 567-571.

Research output: Contribution to journalArticle

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AU - Chan, Paul

AU - Lin, Cheng I.

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N2 - Introduction: Pulmonary veins (PVs) are known to initiate paroxysmal atrial fibrillation. T-type calcium current (ICa-T) has a role in normal and abnormal automaticity of cardiomyocytes. The aim of this study was to evaluate whether ICa-T contributes to PV electrical activity. Methods and Results: By whole-cell clamp techniques in rabbit myocytes, I Ca-T was identified in 12 (39%) of 31 PV cardiomyocytes with pacemaker activity, 2 (9%) of 23 PV cardiomyocytes without pacemaker activity, and 2 (15%) of 13 atrial myocytes (P <0.05). Maximum ICa-L and ICa-T densities from PV cardiomyocytes with pacemaker activity were 6.87 ± 2.17 pA/pF and 1.38 ± 0.69 pA/pF, respectively. Nickel (40 μM) decreased the spontaneous activity in 5 (36%) of 14 PV cardiomyocytes (3.1 ± 0.6 Hz vs 2.2 ± 0.5 Hz, P <0.05), reduced the amplitudes of delayed afterdepolarization from 13 ± 1 mV to 7 ± 1 mV (n = 4, P <0.05) and inhibited transient inward currents from 1.2 ± 0.2 pA/pF to 0.7 ± 0.1 pA/pF (n = 11, P <0.01). Conclusions: We conclude that ICa-T contributes to PV pacemaker activity and triggered activity, which are of functional importance in PV arrhythmogenesis.

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