T lymphocytes and cytokine production in ascitic fluid of ovarian malignancies

Chun Kai Chen, Ming Yih Wu, Kuang Han Chao, Hong Nerng Ho, Bor Ching Sheu, Su Cheng Huang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The activation status oft lymphocytes and the presence of various cytokines in ascitic fluid were examined to test peritoneal immunity in women with ovarian malignancies. Peripheral blood and peritoneal fluid were collected from 12 patients with primary ovarian cancer with ascites and 27 normal control subjects during laparoscopic examination. Lymphocyte subpopulations and the expression of activation markers on T lymphocytes were analyzed by dual-color flow cytometry. The concentrations of various cytokines and soluble interleukin (IL)-2 receptor-α were measured. CD8 T lymphocytes were the main component of peritoneal lymphocytes. CD69 and HLA- DR, but not CD95, were highly expressed on peritoneal T lymphocytes compared to those in peripheral blood. In ascitic fluid of ovarian malignancies, CD4 T lymphocyte concentrations were further decreased, resulting in a decreased CD4/CD8 ratio. Decreased expression of CD69 and CD95 was also noted on T lymphocytes from ascites compared with T lymphocytes in normal peritoneal fluid. IL-1b, tumor necrosis factor-α, IL-6, and soluble IL-2 receptor-α concentrations were increased significantly in the ascitic fluid of women with ovarian cancer. The decrease in activation markers on T lymphocytes is suggestive of an immunosuppressive state, despite the presence of abundant stimulatory cytokines. The immunosuppression may be multifactorial, attributed, in part, to the increased concentrations of soluble IL-9 receptor-α and other inhibitors.

Original languageEnglish
Pages (from-to)24-30
Number of pages7
JournalJournal of the Formosan Medical Association
Volume98
Issue number1
Publication statusPublished - Jan 1 1999
Externally publishedYes

Keywords

  • Activation marker
  • Ascitic fluid T lymphocyte
  • Cytokines
  • Ovarian malignancy
  • Soluble interleukin-2 receptor

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Chen, C. K., Wu, M. Y., Chao, K. H., Ho, H. N., Sheu, B. C., & Huang, S. C. (1999). T lymphocytes and cytokine production in ascitic fluid of ovarian malignancies. Journal of the Formosan Medical Association, 98(1), 24-30.

T lymphocytes and cytokine production in ascitic fluid of ovarian malignancies. / Chen, Chun Kai; Wu, Ming Yih; Chao, Kuang Han; Ho, Hong Nerng; Sheu, Bor Ching; Huang, Su Cheng.

In: Journal of the Formosan Medical Association, Vol. 98, No. 1, 01.01.1999, p. 24-30.

Research output: Contribution to journalArticle

Chen, CK, Wu, MY, Chao, KH, Ho, HN, Sheu, BC & Huang, SC 1999, 'T lymphocytes and cytokine production in ascitic fluid of ovarian malignancies', Journal of the Formosan Medical Association, vol. 98, no. 1, pp. 24-30.
Chen, Chun Kai ; Wu, Ming Yih ; Chao, Kuang Han ; Ho, Hong Nerng ; Sheu, Bor Ching ; Huang, Su Cheng. / T lymphocytes and cytokine production in ascitic fluid of ovarian malignancies. In: Journal of the Formosan Medical Association. 1999 ; Vol. 98, No. 1. pp. 24-30.
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