Synthesized Peptides from Yam Dioscorin Hydrolysis in Silico Exhibit Dipeptidyl Peptidase-IV Inhibitory Activities and Oral Glucose Tolerance Improvements in Normal Mice

Yin Shiou Lin, Chuan Hsiao Han, Shyr Yi Lin, Wen Chi Hou

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

RRDY, RL, and DPF were the top 3 of 21 peptides for inhibitions against dipeptidyl peptidase-IV (DPP-IV) from the pepsin hydrolysis of yam dioscorin in silico and were further investigated in a proof-of-concept study in normal ICR mice for regulating glucose metabolism by the oral glucose tolerance test (OGTT). The sample or sitagliptin (positive control) was orally administered by a feeding gauge; 30 min later, the glucose loads (2.5 g/kg) were performed. RRDY, yam dioscorin, or sitagliptin preload, but not DPF, lowered the area under the curve (AUC0-120) of blood glucose and DPP-IV activity and elevated the AUC0-120 of blood insulin, which showed significant differences compared to control (P < 0.05 or 0.001). These results suggested that RRDY and yam dioscorin might be beneficial in glycemic control in normal mice and need further investigations in diabetic animal models.

Original languageEnglish
Pages (from-to)6451-6458
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume64
Issue number33
DOIs
Publication statusPublished - Aug 24 2016

Keywords

  • blood insulin
  • blood sugar
  • dipeptidyl peptidase-IV (DPP-IV)
  • oral glucose tolerance test (OGTT)
  • sitagliptin
  • yam dioscorin

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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