Synthesis of polysaccharide scaffolds with bioactive substances for tissue engineering

Y. B. Wu, F. L. Mi, C. K. Peng, S. S. Shyu, Sh Yu, M. F. Huang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Based on polysaccharide materials, chitosan-alginate polyelectrolyte complex, are widely used on tissue engineering for wound repair and tissue reconstruction. The goal of study is to combine bioactive substances, basic fibroblast growth factor (bFGF) and mice epidermal growth factor (mEGF), into polysaccharide scaffolds, which serve as controlled-release growth factors carriers to induce vascularization and mediate fibroblast migration. The result indicate that release rate of bFGF could be controlled by varying the pH of alginate solution in the initial incorporation content of bFGF. The revulsive tissue reconstruction of these polysaccharide scaffolds in vivo was evaluated by histological H&E stain and DSC analysis after implantation in SD rat. The vascular was aplenty generated surrounding the bFGF incorporation polysaccharide scaffolds after 1 week of implantation. At 3-week post-operation, new synthesis of collagen was found inside the scaffold, but non-loading bFGF scaffolds were not detected. Further more, the scaffold will be biolysis and replacement by new tissue. All experiment shows that polysaccharide scaffolds incorporate with bFGF may be beneficial to enhance vascularization in the initial time and regeneration new tissue in last stage, which has potential to apply in tissue engineering field.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Pages1512
Number of pages1
Publication statusPublished - 2004
Externally publishedYes
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: May 17 2004May 21 2004

Other

OtherTransactions - 7th World Biomaterials Congress
CountryAustralia
CitySydney
Period5/17/045/21/04

Fingerprint

Fibroblasts
Scaffolds (biology)
Polysaccharides
Tissue engineering
Scaffolds
Alginate
Tissue
Reconstruction (structural)
Tissue regeneration
Bioelectric potentials
Intercellular Signaling Peptides and Proteins
Polyelectrolytes
Chitosan
Collagen
Ion implantation
Rats
Repair
Experiments

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Wu, Y. B., Mi, F. L., Peng, C. K., Shyu, S. S., Yu, S., & Huang, M. F. (2004). Synthesis of polysaccharide scaffolds with bioactive substances for tissue engineering. In Transactions - 7th World Biomaterials Congress (pp. 1512)

Synthesis of polysaccharide scaffolds with bioactive substances for tissue engineering. / Wu, Y. B.; Mi, F. L.; Peng, C. K.; Shyu, S. S.; Yu, Sh; Huang, M. F.

Transactions - 7th World Biomaterials Congress. 2004. p. 1512.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wu, YB, Mi, FL, Peng, CK, Shyu, SS, Yu, S & Huang, MF 2004, Synthesis of polysaccharide scaffolds with bioactive substances for tissue engineering. in Transactions - 7th World Biomaterials Congress. pp. 1512, Transactions - 7th World Biomaterials Congress, Sydney, Australia, 5/17/04.
Wu YB, Mi FL, Peng CK, Shyu SS, Yu S, Huang MF. Synthesis of polysaccharide scaffolds with bioactive substances for tissue engineering. In Transactions - 7th World Biomaterials Congress. 2004. p. 1512
Wu, Y. B. ; Mi, F. L. ; Peng, C. K. ; Shyu, S. S. ; Yu, Sh ; Huang, M. F. / Synthesis of polysaccharide scaffolds with bioactive substances for tissue engineering. Transactions - 7th World Biomaterials Congress. 2004. pp. 1512
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