Synthesis of a novel glycoconjugated chitosan and preparation of its derived nanoparticles for targeting HepG2 cells

Fwu Long Mi, Yong Yi Wu, Ya Lin Chiu, Mei Chin Chen, Hsing Wen Sung, Shu Huei Yu, Shin Shing Shyu, Mei Feng Huang

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

In the study, a novel chitosan (CS) derivative conjugated with multiple galactose residues in an antennary fashion (Gal-m-CS) was synthesized. A galactosylated CS (Gal-CS) was also prepared by directly coupling lactobionic acid on CS. Using an iontropic gelation method, CS and the synthesized Gal-CS and Gal-m-CS were used to prepare nanoparticles (CS, Gal-CS, and Gal-m-CS NPs) for targeting hepatoma cells. TEM examinations showed that the morphology of all three types of NPs was spherical in shape. No aggregation or precipitation of NPs in an aqueous environment was observed during storage for all studied groups, as a result of the electrostatic repulsion between the positively charged NPs. Little fluorescence was observed in HepG2 cells after incubation with the FITC-labeled CS NPs. The intensity of fluorescence observed in HepG2 cells incubated with the Gal-m-CS NPs was stronger than that incubated with the Gal-CS NPs. These results indicated that the prepared Gal-m-CS NPs had the highest specific interaction with HepG2 cells among all studied groups, via the ligand-receptor-mediated recognition.

Original languageEnglish
Pages (from-to)892-898
Number of pages7
JournalBiomacromolecules
Volume8
Issue number3
DOIs
Publication statusPublished - Mar 2007
Externally publishedYes

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Hep G2 Cells
Chitosan
Nanoparticles
Fluorescence
Fluorescein-5-isothiocyanate
Gelation
Static Electricity
Galactose
Hepatocellular Carcinoma
Electrostatics
Agglomeration
Ligands
Transmission electron microscopy
Derivatives

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Synthesis of a novel glycoconjugated chitosan and preparation of its derived nanoparticles for targeting HepG2 cells. / Mi, Fwu Long; Wu, Yong Yi; Chiu, Ya Lin; Chen, Mei Chin; Sung, Hsing Wen; Yu, Shu Huei; Shyu, Shin Shing; Huang, Mei Feng.

In: Biomacromolecules, Vol. 8, No. 3, 03.2007, p. 892-898.

Research output: Contribution to journalArticle

Mi, Fwu Long ; Wu, Yong Yi ; Chiu, Ya Lin ; Chen, Mei Chin ; Sung, Hsing Wen ; Yu, Shu Huei ; Shyu, Shin Shing ; Huang, Mei Feng. / Synthesis of a novel glycoconjugated chitosan and preparation of its derived nanoparticles for targeting HepG2 cells. In: Biomacromolecules. 2007 ; Vol. 8, No. 3. pp. 892-898.
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AU - Chen, Mei Chin

AU - Sung, Hsing Wen

AU - Yu, Shu Huei

AU - Shyu, Shin Shing

AU - Huang, Mei Feng

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