Synthesis and characterization of Gd-DTPA/fucoidan/peptide complex nanoparticle and in vitro magnetic resonance imaging of inflamed endothelial cells

Tsai Mu Cheng, Rou Li, Yu Chieh Jill Kao, Chun Hua Hsu, Hsueh Liang Chu, Kun Ying Lu, Chun A. Changou, Che Chang Chang, Lee Hsin Chang, Min Lang Tsai, Fwu Long Mi

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1 Citation (Scopus)

Abstract

P-selectin overexpressed on activated endothelial cells and platelets is a new target for treatment of cancers and cardiovascular diseases such as atherosclerosis and thrombosis. In this study, depolymerized low molecular weight fucoidan (LMWF8775) and a thermolysin-hydrolyzed protamine peptide (TPP1880) were prepared. TPP1880 and LMWF8775 were able to form self-assembled complex nanoparticles (CNPs). The formation of TPP1880/LMWF8775 CNPs was characterized by Fourier-transform infrared spectra, circular dichroism spectra and isothermal titration calorimetry. The CNPs selectively targeted PMA-stimulated, inflamed endothelial cells (HUVECs) with high expression of P-selectin. Gd-DTPA MRI contrast agent was successfully loaded in the CNPs with better T1 relaxivity and selectively accumulated in the activated HUVECs with increased MRI intensity and reduced cytotoxicity as compared to free Gd-DTPA. Our results suggest that the TPP1880/LMWF8775 CNPs may have potential in future for early diagnosis of cardiovascular diseases and cancers in which the endothelium is inflamed or activated.

Original languageEnglish
Article number111064
JournalMaterials Science and Engineering C
Volume114
DOIs
Publication statusPublished - Sep 2020

Keywords

  • Cell penetrating peptide
  • Drug delivery
  • Fucoidan
  • Imaging
  • Nanoparticles
  • Targeting

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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