Synthesis and characterization of a novel chitosan-gelatin bioconjugate with fluorescence emission

Research output: Contribution to journalArticle

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Abstract

Polysaccharide-protein conjugations have generated increasing interests for biomedical applications in recent years. A naturally occurring cross-linking reagent, genipin, which has been used in herbal medicine, was employed to cross-link chitosan and gelatin for the preparation of a novel chitosan-gelatin conjugate. The primary amine groups on chitosan and gelatin were covalently linked with genipin, leading to the formation of a chitosan-gelatin conjugate with nitrogen-containing heterocycle units, the pyrindine-like derivatives. The FT-IR and UV-vis studies revealed that chitosan could react with genipin via a nucleophilic ring-opening reaction to construct more sufficient and extensive cross-link networks, as compared with its gelatin counterpart. The UV-vis absorption properties of the chitosan-gelatin conjugates were strongly related to the chitosan-to-gelatin weight ratio in the compositions. It is worth noting that the conjugation process endows the special emission properties of the chitosan-gelatin conjugates, which depends on the cross-linking reaction and the formation of hydrogen bonding involved chitosan-gelatin complex. Fluorescence quenching or enhancement was observed from the chitosan-gelatin conjugates upon coordinated with a wide variety of heavy metal ions (Ag+, CU2+, Fe2+, and Co2+). This study also examined the possibility of covalent coupling the capture chelator (chitosan) with bioactive protein (e.g., albumin, α-globulin, and fibrinogen) to create fluorescence emission. These findings may provide a novel way to deliver therapeutic radionuclides for immune-targeting purposes in the future.

Original languageEnglish
Pages (from-to)975-987
Number of pages13
JournalBiomacromolecules
Volume6
Issue number2
DOIs
Publication statusPublished - Mar 2005
Externally publishedYes

Fingerprint

Chitosan
Gelatin
Fluorescence
Cross-Linking Reagents
Proteins
Heavy Ions
Herbal Medicine
Cross Reactions
Globulins
Hydrogen Bonding
Polysaccharides
Chelating Agents
Heavy Metals
Heavy ions
Radioisotopes
Fibrinogen
Heavy metals
Amines
Metal ions
Albumins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics

Cite this

Synthesis and characterization of a novel chitosan-gelatin bioconjugate with fluorescence emission. / Mi, Fwu Long.

In: Biomacromolecules, Vol. 6, No. 2, 03.2005, p. 975-987.

Research output: Contribution to journalArticle

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