SYNTHESIS AND BIOLOGICAL ACTIVITY OF HUMAN β‐ENDORPHIN ANALOGS WITH DISULFIDE BRIDGES

JAMES BLAKE, WEN‐CHANG ‐C CHANG, CHOH HAO LI

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Three analogs of human β‐endorphin containing internal cystine bridges have been synthesized and their biological activities have been determined. It was found that the analogs with disulfide bridges between positions 17 and 26 or 11 and 26 retained full opiate activity as estimated by guinea ileum assay, whereas the analog with a cystine bridge between positions 7 and 26 showed reduced opiate activity. None of the analogs retained significant immunoactivity as revealed by radioimmunoassay.

Original languageEnglish
Pages (from-to)275-280
Number of pages6
JournalInternational Journal of Peptide and Protein Research
Volume14
Issue number3
DOIs
Publication statusPublished - Jan 1 1979
Externally publishedYes

Fingerprint

Opiate Alkaloids
Endorphins
Cystine
Bioactivity
Human Activities
Disulfides
Guinea
Ileum
Radioimmunoassay
Assays

Keywords

  • disulfide bridges
  • opiate activity
  • radioimmunoactivity
  • β‐endorphin

ASJC Scopus subject areas

  • Biochemistry

Cite this

SYNTHESIS AND BIOLOGICAL ACTIVITY OF HUMAN β‐ENDORPHIN ANALOGS WITH DISULFIDE BRIDGES. / BLAKE, JAMES; CHANG, WEN‐CHANG ‐C; LI, CHOH HAO.

In: International Journal of Peptide and Protein Research, Vol. 14, No. 3, 01.01.1979, p. 275-280.

Research output: Contribution to journalArticle

@article{f0df046809114ed09d8fa1e7e076dafe,
title = "SYNTHESIS AND BIOLOGICAL ACTIVITY OF HUMAN β‐ENDORPHIN ANALOGS WITH DISULFIDE BRIDGES",
abstract = "Three analogs of human β‐endorphin containing internal cystine bridges have been synthesized and their biological activities have been determined. It was found that the analogs with disulfide bridges between positions 17 and 26 or 11 and 26 retained full opiate activity as estimated by guinea ileum assay, whereas the analog with a cystine bridge between positions 7 and 26 showed reduced opiate activity. None of the analogs retained significant immunoactivity as revealed by radioimmunoassay.",
keywords = "disulfide bridges, opiate activity, radioimmunoactivity, β‐endorphin",
author = "JAMES BLAKE and CHANG, {WEN‐CHANG ‐C} and LI, {CHOH HAO}",
year = "1979",
month = "1",
day = "1",
doi = "10.1111/j.1399-3011.1979.tb01933.x",
language = "English",
volume = "14",
pages = "275--280",
journal = "International Journal of Peptide and Protein Research",
issn = "0367-8377",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - SYNTHESIS AND BIOLOGICAL ACTIVITY OF HUMAN β‐ENDORPHIN ANALOGS WITH DISULFIDE BRIDGES

AU - BLAKE, JAMES

AU - CHANG, WEN‐CHANG ‐C

AU - LI, CHOH HAO

PY - 1979/1/1

Y1 - 1979/1/1

N2 - Three analogs of human β‐endorphin containing internal cystine bridges have been synthesized and their biological activities have been determined. It was found that the analogs with disulfide bridges between positions 17 and 26 or 11 and 26 retained full opiate activity as estimated by guinea ileum assay, whereas the analog with a cystine bridge between positions 7 and 26 showed reduced opiate activity. None of the analogs retained significant immunoactivity as revealed by radioimmunoassay.

AB - Three analogs of human β‐endorphin containing internal cystine bridges have been synthesized and their biological activities have been determined. It was found that the analogs with disulfide bridges between positions 17 and 26 or 11 and 26 retained full opiate activity as estimated by guinea ileum assay, whereas the analog with a cystine bridge between positions 7 and 26 showed reduced opiate activity. None of the analogs retained significant immunoactivity as revealed by radioimmunoassay.

KW - disulfide bridges

KW - opiate activity

KW - radioimmunoactivity

KW - β‐endorphin

UR - http://www.scopus.com/inward/record.url?scp=0018703551&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018703551&partnerID=8YFLogxK

U2 - 10.1111/j.1399-3011.1979.tb01933.x

DO - 10.1111/j.1399-3011.1979.tb01933.x

M3 - Article

C2 - 521210

AN - SCOPUS:0018703551

VL - 14

SP - 275

EP - 280

JO - International Journal of Peptide and Protein Research

JF - International Journal of Peptide and Protein Research

SN - 0367-8377

IS - 3

ER -