Synergy of endothelial and neural progenitor cells from adipose-derived stem cells to preserve neurovascular structures in rat hypoxic-ischemic brain injury

Yuan Yu Hsueh, Ya Ju Chang, Chia Wei Huang, Fitri Handayani, Yi Lun Chiang, Shih Chen Fan, Chien Jung Ho, Yu Min Kuo, Shang Hsun Yang, Yuh Ling Chen, Sheng Che Lin, Chao Ching Huang, Chia Ching Wu

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Abstract

Perinatal cerebral hypoxic-ischemic (HI) injury damages the architecture of neurovascular units (NVUs) and results in neurological disorders. Here, we differentiated adipose-derived stem cells (ASCs) toward the progenitor of endothelial progenitor cells (EPCs) and neural precursor cells (NPCs) via microenvironmental induction and investigated the protective effect by transplanting ASCs, EPCs, NPCs, or a combination of EPCs and NPCs (E+N) into neonatal HI injured rat pups. The E+N combination produced significant reduction in brain damage and cell apoptosis and the most comprehensive restoration in NVUs regarding neuron number, normal astrocytes, and vessel density. Improvements in cognitive and motor functions were also achieved in injured rats with E+N therapy. Synergistic interactions to facilitate transmigration under in vitro hypoxic microenvironment were discovered with involvement of the neuropilin-1 (NRP1) signal in EPCs and the C-X-C chemokine receptor 4 (CXCR4) and fibroblast growth factor receptor 1 (FGFR1) signals in NPCs. Therefore, ASCs exhibit great potential for cell sources in endothelial and neural lineages to prevent brain from HI damage.

Original languageEnglish
Article number14985
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - Oct 8 2015

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Brain Injuries
Stem Cells
Neuropilin-1
Receptor, Fibroblast Growth Factor, Type 1
CXC Chemokines
Brain Hypoxia
Chemokine Receptors
Nervous System Diseases
Astrocytes
Cognition
Endothelial Progenitor Cells
Apoptosis
Neurons
Wounds and Injuries
Brain

ASJC Scopus subject areas

  • General

Cite this

Synergy of endothelial and neural progenitor cells from adipose-derived stem cells to preserve neurovascular structures in rat hypoxic-ischemic brain injury. / Hsueh, Yuan Yu; Chang, Ya Ju; Huang, Chia Wei; Handayani, Fitri; Chiang, Yi Lun; Fan, Shih Chen; Ho, Chien Jung; Kuo, Yu Min; Yang, Shang Hsun; Chen, Yuh Ling; Lin, Sheng Che; Huang, Chao Ching; Wu, Chia Ching.

In: Scientific Reports, Vol. 5, 14985, 08.10.2015.

Research output: Contribution to journalArticle

Hsueh, YY, Chang, YJ, Huang, CW, Handayani, F, Chiang, YL, Fan, SC, Ho, CJ, Kuo, YM, Yang, SH, Chen, YL, Lin, SC, Huang, CC & Wu, CC 2015, 'Synergy of endothelial and neural progenitor cells from adipose-derived stem cells to preserve neurovascular structures in rat hypoxic-ischemic brain injury', Scientific Reports, vol. 5, 14985. https://doi.org/10.1038/srep14985
Hsueh, Yuan Yu ; Chang, Ya Ju ; Huang, Chia Wei ; Handayani, Fitri ; Chiang, Yi Lun ; Fan, Shih Chen ; Ho, Chien Jung ; Kuo, Yu Min ; Yang, Shang Hsun ; Chen, Yuh Ling ; Lin, Sheng Che ; Huang, Chao Ching ; Wu, Chia Ching. / Synergy of endothelial and neural progenitor cells from adipose-derived stem cells to preserve neurovascular structures in rat hypoxic-ischemic brain injury. In: Scientific Reports. 2015 ; Vol. 5.
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