Synergistic Impact of Nicotine and Shear Stress Induces Cytoskeleton Collapse and Apoptosis in Endothelial Cells

Yu Hsiang Lee, Ruei Siang Chen, Nen Chung Chang, Kueir Rarn Lee, Chien Tsai Huang, Yu Ching Huang, Feng Ming Ho

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Nicotine is the major component in cigarette smoke and has been recognized as a risk factor for various cardiovascular diseases such as atherosclerosis. However, the definite pathogenesis of nicotine-mediated endothelial dysfunction remains unclear because hemodynamic factor in most of prior in vitro studies was excluded. To understand how nicotine affects endothelium in the dynamic environment, human umbilical vein endothelial cells were treated by different laminar shear stresses (LSS; 0, 6, 8, and 12 dynes cm−2) with and without 10−4 M nicotine for 12 h in a parallel plate flow system, following detections of cellular morphology and apoptotic level. Our results showed that cells sheared by 12 dynes cm−2 LSS with nicotine excessively elongated and aligned with the flow direction, and exhibited significant apoptosis as compared to the groups with nicotine or LSS alone. We reasoned that the irregular morphological rearrangement and elevated apoptosis were resulted from the interruption of mechanostasis due to cytoskeletal collapse. Furthermore, all the impaired responses can be rescued by treatment with free radical scavenger ascorbic acid (10−4 M), indicating oxidative stress was likely mediated with the impairments. In summary, our findings demonstrated an essential role of LSS in nicotine-mediated endothelial injury occurring in the physiological environment.

Original languageEnglish
Pages (from-to)2220-2230
Number of pages11
JournalAnnals of Biomedical Engineering
Volume43
Issue number9
DOIs
Publication statusPublished - Sep 29 2015

Fingerprint

Nicotine
Endothelial cells
Cell death
Shear stress
Oxidative stress
Ascorbic acid
Hemodynamics
Free radicals
Smoke

Keywords

  • Apoptosis
  • Cytoskeleton disintegration
  • Laminar shear stress
  • Morphological reorganization
  • Nicotine

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Synergistic Impact of Nicotine and Shear Stress Induces Cytoskeleton Collapse and Apoptosis in Endothelial Cells. / Lee, Yu Hsiang; Chen, Ruei Siang; Chang, Nen Chung; Lee, Kueir Rarn; Huang, Chien Tsai; Huang, Yu Ching; Ho, Feng Ming.

In: Annals of Biomedical Engineering, Vol. 43, No. 9, 29.09.2015, p. 2220-2230.

Research output: Contribution to journalArticle

Lee, Yu Hsiang ; Chen, Ruei Siang ; Chang, Nen Chung ; Lee, Kueir Rarn ; Huang, Chien Tsai ; Huang, Yu Ching ; Ho, Feng Ming. / Synergistic Impact of Nicotine and Shear Stress Induces Cytoskeleton Collapse and Apoptosis in Endothelial Cells. In: Annals of Biomedical Engineering. 2015 ; Vol. 43, No. 9. pp. 2220-2230.
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