Synergistic hierarchical silicone-modified polysaccharide hybrid as a soft scaffold to control cell adhesion and proliferation

Wei Chen Huang, Kun Ho Liu, Ta Chung Liu, Dean Mo Liu, San Yuan Chen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In this study, a new type of polydimethylsiloxane-modified chitosan (PMSC) amphiphilic hydrogel was developed as a soft substrate to explore cellular responses for dermal reconstruction. The hydrogel wettability, mechanical stiffness and topography were controllable through manipulation of the degree of esterification (DE) between hydrophobic polydimethylsiloxane (PDMS) and hydrophilic N,O-(carboxymethyl)-chitosan (NOCC). Based on microphase separation, the incorporation of PDMS into NOCC increased the stiffness of the hybrid through the formation of self-assembled aggregates, which also provided anchor sites for cell adhesion. As the DE exceeded 0.39, the size of the PDMS-rich aggregates changed from nanoscale to microscale. Subsequently, the hierarchical architecture resulted in an increase in the tensile modulus of the hybrid gel up to fourfold, which simultaneously provided mechano-topographic guidance and allowed the cells to completely spread to form spindle shapes instead of forming a spherical morphology, as on NOCC (DE = 0). The results revealed that the incorporation of hydrophobic PDMS not only impeded acidic damage resulting from NOCC but also acted as an adhesion modification agent to facilitate long-term cell adhesion and proliferation on the soft substrate. As proved by the promotion on long-term type-I collagen production, the PMSC hybrid with self-assembled mechano-topography offers great promise as an advanced scaffold material for use in healing applications.

Original languageEnglish
Pages (from-to)3546-3556
Number of pages11
JournalActa Biomaterialia
Volume10
Issue number8
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Cell adhesion
Cell proliferation
Silicones
Polydimethylsiloxane
Scaffolds (biology)
Polysaccharides
Chitosan
Cell Adhesion
Cell Proliferation
Esterification
Hydrogel
Hydrogels
Topography
Stiffness
Wettability
Microphase separation
Substrates
Collagen Type I
Anchors
Collagen

Keywords

  • Amphiphilic copolymer
  • Cell adhesion
  • Interface manipulation
  • Mechanical stiffness
  • Topography

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Medicine(all)

Cite this

Synergistic hierarchical silicone-modified polysaccharide hybrid as a soft scaffold to control cell adhesion and proliferation. / Huang, Wei Chen; Liu, Kun Ho; Liu, Ta Chung; Liu, Dean Mo; Chen, San Yuan.

In: Acta Biomaterialia, Vol. 10, No. 8, 2014, p. 3546-3556.

Research output: Contribution to journalArticle

Huang, Wei Chen ; Liu, Kun Ho ; Liu, Ta Chung ; Liu, Dean Mo ; Chen, San Yuan. / Synergistic hierarchical silicone-modified polysaccharide hybrid as a soft scaffold to control cell adhesion and proliferation. In: Acta Biomaterialia. 2014 ; Vol. 10, No. 8. pp. 3546-3556.
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