Abstract

Osteoarthritis (OA) is a common disease associated with tissue inflammation, physical disability and imbalanced homeostasis in cartilage. For advanced treatments, biological approaches are currently focused on tissue regeneration and anti-inflammation. This study was undertaken to evaluate the therapeutic efficacies of hyaluronic acid (HA) and platelet-rich plasma (PRP) (HA+PRP) on OA. Articular chondrocytes were obtained from five OA patients. The optimal HA and PRP concentrations were evaluated by MTT assay. The expressions of chondrogenic and inflammatory genes were analyzed by RT-PCR. Signaling pathway was examined by immunoblotting and the expressions of OA pathology-related chemokines and cytokines was demonstrated by real-time PCR-based SuperArray. The therapeutic efficacies of HA+PRP were then demonstrated in 3D arthritic neo-cartilage and ACLT-OA model. Here we showed that HA+PRP could greatly retrieve pro-inflammatory cytokines-reduced articular chondrocytes proliferation and chondrogenic phenotypes, the mechanism of which involve the sequential activation of specific receptors CD44 and TGF-βRII, downstream mediators Smad2/3 and Erk1/2, and the chondrogenic transcription factor SOX9. The real-time PCR-based SuperArray results also indicated that OA pathology-related chemokines and cytokines could be efficiently suppressed by HA+PRP. Moreover, the cartilaginous ECM could be retrieved from inflammation-induced degradation by HA+PRP in both 2D monolayer and 3D neo-cartilage model. Finally, the intra-articular injection of HA+PRP could strongly rescue the meniscus tear and cartilage breakdown and then decrease OA-related immune cells. The combination of HA+PRP can synergistically promote cartilage regeneration and inhibit OA inflammation. This study might offer an advanced and alternative OA treatment based on detailed regenerative mechanisms.

Original languageEnglish
Pages (from-to)9599-9607
Number of pages9
JournalBiomaterials
Volume35
Issue number36
DOIs
Publication statusPublished - 2014

Fingerprint

Hyaluronic acid
Platelet-Rich Plasma
Cartilage
Hyaluronic Acid
Platelets
Osteoarthritis
Regeneration
Plasmas
Inflammation
Therapeutics
Pathology
Cytokines
Chondrocytes
Chemokines
SOX9 Transcription Factor
Real-Time Polymerase Chain Reaction
Joints
Military electronic countermeasures
Intra-Articular Injections
Tissue regeneration

Keywords

  • Anti-inflammation
  • Hyaluronic acid (HA)
  • Osteoarthritis (OA)
  • Platelet-rich plasma (PRP)
  • Tissue regeneration

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics
  • Medicine(all)

Cite this

Synergistic anabolic actions of hyaluronic acid and platelet-rich plasma on cartilage regeneration in osteoarthritis therapy. / Chen, Wei Hong; Lo, Wen-Cheng; Hsu, Wei Che; Wei, Hong Jian; Liu, Hen Yu; Lee, Chian-Her; Tina Chen, Szu Yu; Shieh, Ying-Hua; Williams, David F.; Deng, Win-Ping.

In: Biomaterials, Vol. 35, No. 36, 2014, p. 9599-9607.

Research output: Contribution to journalArticle

Chen, Wei Hong ; Lo, Wen-Cheng ; Hsu, Wei Che ; Wei, Hong Jian ; Liu, Hen Yu ; Lee, Chian-Her ; Tina Chen, Szu Yu ; Shieh, Ying-Hua ; Williams, David F. ; Deng, Win-Ping. / Synergistic anabolic actions of hyaluronic acid and platelet-rich plasma on cartilage regeneration in osteoarthritis therapy. In: Biomaterials. 2014 ; Vol. 35, No. 36. pp. 9599-9607.
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AU - Lo, Wen-Cheng

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AU - Wei, Hong Jian

AU - Liu, Hen Yu

AU - Lee, Chian-Her

AU - Tina Chen, Szu Yu

AU - Shieh, Ying-Hua

AU - Williams, David F.

AU - Deng, Win-Ping

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