Synergism of biochemical and mechanical stimuli in the differentiation of human placenta-derived multipotent cells into endothelial cells

Chia Ching Wu, You Chen Chao, Cheng Nan Chen, Shu Chien, Yao Chang Chen, Chih Chung Chien, Jeng Jiann Chiu, B. Linju Yen

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

There have been intensive studies on the differentiation of endothelial progenitor cells (EPCs) into endothelial cells. We investigated the endothelial differentiation of placenta-derived multipotent cells (PDMCs), a population of CD34-/CD133-/Flk-1- cells. PDMCs were cultured in basal media or media containing endothelial growth factors (EGM), including vascular endothelial growth factor (VEGF), for 3 days and then subjected to shear stress of 6 or 12 dyn/cm2 for 24 h. Culture of PDMCs in EGM under static conditions resulted in significant increases in VEGF receptor-1 (Flt-1) and receptor-2 (Flk-1) expression. Application of shear stress at 12 dyn/cm2 to these cells led to significant increases in their expression of von Willebrand Factor and platelet-endothelial cell adhesion molecule-1 at both the gene and protein levels. Shear stress at 6 dyn/cm2 had lesser effects. Uptakes of acetylated low-density lipoproteins as well as formation of tube-like structures on Matrigel were significantly increased after subjecting to shear stress of 12 dyn/cm2 for 24 h. Our findings suggest that the combined use of endothelial growth factors and high shear stress is synergistic for the endothelial differentiation of PDMCs.

Original languageEnglish
Pages (from-to)813-821
Number of pages9
JournalJournal of Biomechanics
Volume41
Issue number4
DOIs
Publication statusPublished - Jan 10 2008
Externally publishedYes

Keywords

  • Endothelial differentiation
  • Placenta
  • Shear flow
  • Stem cell

ASJC Scopus subject areas

  • Biophysics
  • Orthopedics and Sports Medicine
  • Biomedical Engineering
  • Rehabilitation

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