Sweet potato (Ipomoea batatas (L.) Lam. 'Tainong 57') storage root mucilage exhibited angiotensin converting enzyme inhibitory activity in vitro

Dong Jiann Huang, Wen Chi Hou, Hsien Jung Chen, Yaw Huei Lin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Sweet potato (Ipomoea batatas (L.) Lam. 'Tainong 57') storage root mucilage was extracted (crude mucilage) and further purified by SDS and heating treatment (purified mucilage). Purified mucilage treated with 2-mercaptoethanol moved as a single band in SDS-PAGE. This purified mucilage was active to inhibit angiotensin converting enzyme (ACE) as shown by spectrophotometric method in a dose-dependent manner (28.7 to 59.8% ACE inhibition, respectively, by 50 to 400 μg/mL mucilage) using (N-(3-[2-furyl] acryloyl)-Phe-Gly-Gly) (FAPGG) as a substrate. The concentration of mucilage required for 50% inhibition (IC 50) of ACE activity was 364.5 μg/mL while that of captopril was 10 nM (8.68 μg/mL). The commercial polysaccharide pectin (50 to 400 μg/mL) showed no inhibitory activity against ACE. When using fluorescent silica TLC to detect FAPGG and FAP, the results also showed that mucilage inhibited ACE. The mucilage showed mixed type inhibition against ACE, and the Michaelis constant in the presence of mucilage was 12 mM. We suggest that consumption of sweet potato storage root mucilage may benefit people's health.

Original languageEnglish
Pages (from-to)397-402
Number of pages6
JournalBotanical Studies
Volume47
Issue number4
Publication statusPublished - Oct 2006

Fingerprint

Ipomoea batatas
mucilages
peptidyl-dipeptidase A
sweet potatoes
enzyme inhibition
silica
pectins
inhibitory concentration 50
polysaccharides
enzyme activity
heat

Keywords

  • Angiotensin converting enzyme (ACE)
  • Mucilage
  • Sweet potato

ASJC Scopus subject areas

  • Plant Science

Cite this

Sweet potato (Ipomoea batatas (L.) Lam. 'Tainong 57') storage root mucilage exhibited angiotensin converting enzyme inhibitory activity in vitro. / Huang, Dong Jiann; Hou, Wen Chi; Chen, Hsien Jung; Lin, Yaw Huei.

In: Botanical Studies, Vol. 47, No. 4, 10.2006, p. 397-402.

Research output: Contribution to journalArticle

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