Surfactant lavage decreases systemic interleukin-1β production in meconium aspiration syndrome

Pei Wei Wang, Mei Jy Jeng, Liang Shun Wang, Li Jung Fang, Wen Jue Soong

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Surfactant lavage has been used to remove meconium debris in meconium aspiration syndrome (MAS), but the influence of surfactant lavage on pro-inflammatory cytokines and cellular apoptosis is unclear. The aim of this study was to investigate the response of pro-inflammatory cytokine and the influence on alveolar cellular apoptosis using therapeutic bronchoalveolar lavage with diluted surfactant to treat MAS. Methods: Twelve newborn piglets were anesthetized, intubated via tracheostomy, and artificially ventilated. MAS was induced by intratracheal instillation of 3-5 mL/kg of 20% human meconium. The piglets were then randomly assigned to a surfactant lavage group (n = 6) or a control group (n = 6). Piglets in the lavage group received bronchoalveolar lavage with 30 mL/kg diluted surfactant (5 mg/mL) in two aliquots. Cardiopulmonary parameters were monitored continuously. Serum was obtained hourly to measure concentrations of pro-inflammatory cytokines, including interleukin (IL)-Iβ, IL-6, and tumor necrosis factor α. Lung tissue was histologically examined after experiments, and terminal deoxynucleotidyl transferase-mediated nick-end labeling assay for apoptotic cell death was also performed. Results: The animals in the lavage group displayed significantly better gas exchange and lower serum concentrations of IL-1β than the animals in the control group (P <0.05). The number of apoptotic cells in lung tissues was significantly lower in the lavage group than the control group, and also in the nondependent than the dependent site. Conclusion: Therapeutic surfactant lavage improves oxygenation, decreases production of systemic pro-inflammatory cytokine IL-1β, and alleviates the severity of lung cell apoptosis in newborn piglets with experimentally-induced MAS.

Original languageEnglish
Pages (from-to)432-437
Number of pages6
JournalPediatrics International
Volume52
Issue number3
DOIs
Publication statusPublished - Jun 2010
Externally publishedYes

Fingerprint

Meconium Aspiration Syndrome
Therapeutic Irrigation
Interleukin-1
Surface-Active Agents
Cytokines
Meconium
Bronchoalveolar Lavage
Apoptosis
Lung
Control Groups
DNA Nucleotidylexotransferase
Tracheostomy
Serum
Interleukin-6
Cell Death
Tumor Necrosis Factor-alpha
Cell Count
Gases
Therapeutics

Keywords

  • Apoptosis
  • Bronchoalveolar lavage
  • Cytokine
  • Meconium aspiration syndrome
  • Surfactant

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Surfactant lavage decreases systemic interleukin-1β production in meconium aspiration syndrome. / Wang, Pei Wei; Jeng, Mei Jy; Wang, Liang Shun; Fang, Li Jung; Soong, Wen Jue.

In: Pediatrics International, Vol. 52, No. 3, 06.2010, p. 432-437.

Research output: Contribution to journalArticle

Wang, Pei Wei ; Jeng, Mei Jy ; Wang, Liang Shun ; Fang, Li Jung ; Soong, Wen Jue. / Surfactant lavage decreases systemic interleukin-1β production in meconium aspiration syndrome. In: Pediatrics International. 2010 ; Vol. 52, No. 3. pp. 432-437.
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abstract = "Background: Surfactant lavage has been used to remove meconium debris in meconium aspiration syndrome (MAS), but the influence of surfactant lavage on pro-inflammatory cytokines and cellular apoptosis is unclear. The aim of this study was to investigate the response of pro-inflammatory cytokine and the influence on alveolar cellular apoptosis using therapeutic bronchoalveolar lavage with diluted surfactant to treat MAS. Methods: Twelve newborn piglets were anesthetized, intubated via tracheostomy, and artificially ventilated. MAS was induced by intratracheal instillation of 3-5 mL/kg of 20{\%} human meconium. The piglets were then randomly assigned to a surfactant lavage group (n = 6) or a control group (n = 6). Piglets in the lavage group received bronchoalveolar lavage with 30 mL/kg diluted surfactant (5 mg/mL) in two aliquots. Cardiopulmonary parameters were monitored continuously. Serum was obtained hourly to measure concentrations of pro-inflammatory cytokines, including interleukin (IL)-Iβ, IL-6, and tumor necrosis factor α. Lung tissue was histologically examined after experiments, and terminal deoxynucleotidyl transferase-mediated nick-end labeling assay for apoptotic cell death was also performed. Results: The animals in the lavage group displayed significantly better gas exchange and lower serum concentrations of IL-1β than the animals in the control group (P <0.05). The number of apoptotic cells in lung tissues was significantly lower in the lavage group than the control group, and also in the nondependent than the dependent site. Conclusion: Therapeutic surfactant lavage improves oxygenation, decreases production of systemic pro-inflammatory cytokine IL-1β, and alleviates the severity of lung cell apoptosis in newborn piglets with experimentally-induced MAS.",
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