Surface charge effect in intracellular localization of mesoporous silica nanoparticles as probed by fluorescent ratiometric pH imaging

Yi-Ping Chen, Hsueh An Chen, Yann Hung, Fan Ching Chien, Peilin Chen, Chung Yuan Mou

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

Mesoporous silica nanoparticles (MSNs) have received intensive recent interest as multi-functional nanocarriers for biological cells and tissues. Their cell uptake and intracellular distribution depends on the surface charge they carry. We develop a new approach for monitoring their uptake and intracellular localization by measuring the local pH value of the MSNs. We synthesized a ratiometric pH-sensor loaded with mesoporous silica nanoparticles that can map the location of the MSN through the site pH in HeLa cells. Positively charged MSN-TA was found to locate in higher pH regions which are identified as cytosol mostly. On the other hand, negatively charged MSN-PPs were trapped in acidic endosomes. Evidence is given for direct penetration of membranes through a charge-mediated membrane-nanoparticle interaction mechanism.

Original languageEnglish
Pages (from-to)968-973
Number of pages6
JournalRSC Advances
Volume2
Issue number3
DOIs
Publication statusPublished - Feb 7 2012
Externally publishedYes

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

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