Surface antigens contribute differently to the pathophysiological features in serotype K1 and K2 Klebsiella pneumoniae strains isolated from liver abscesses

Kuo Ming Yeh, Sheng Kung Chiu, Chii Lan Lin, Li Yueh Huang, Yu Kuo Tsai, Jen Chang Chang, Jung Chung Lin, Feng Yee Chang, Leung Kei Siu

Research output: Contribution to journalArticle

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Abstract

Background: The virulence role of surface antigens in a single serotype of Klebsiella pneumoniae strain have been studied, but little is known about whether their contribution will vary with serotype. Method: To investigate the role of K and O antigen in hyper-virulent strains, we constructed O and K antigen deficient mutants from serotype K1 STL43 and K2 TSGH strains from patients with liver abscess, and characterized their virulence in according to the abscess formation and resistance to neutrophil phagocytosis, serum, and bacterial clearance in liver. Results: Both of K1 and K2-antigen mutants lost their wildtype resistance to neutrophil phagocytosis and hepatic clearance, and failed to cause abscess formation. K2-antigen mutant became serum susceptible while K1-antigen mutant maintained its resistance to serum killing. The amount of glucuronic acid, indicating the amount of capsular polysaccharide (CPS, K antigen), was inversed proportional to the rate of phagocytosis. O-antigen mutant of serotype K1 strains had significantly more amount of CPS, and more resistant to neutrophil phagocytosis than its wildtype counterpart. O-antigen mutants of serotype K1 and K2 strains lost their wildtype serum resistance, and kept resistant to neutrophil phagocytosis. While both mutants lacked the same O1 antigen, O-antigen mutant of serotype K1 became susceptible to liver clearance and cause mild abscess formation, but its serotype K2 counterpart maintained these wildtype virulence. Conclusion: We conclude that the contribution of surface antigens to virulence of K. pneumoniae strains varies with serotypes.

Original languageEnglish
Article number4
JournalGut Pathogens
Volume8
Issue number1
DOIs
Publication statusPublished - Feb 13 2016

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Liver Abscess
Klebsiella pneumoniae
Surface Antigens
O Antigens
Phagocytosis
Virulence
Neutrophils
Abscess
Antigens
Serum
Liver
Glucuronic Acid
Serogroup

ASJC Scopus subject areas

  • Virology
  • Gastroenterology
  • Infectious Diseases
  • Microbiology
  • Parasitology

Cite this

Surface antigens contribute differently to the pathophysiological features in serotype K1 and K2 Klebsiella pneumoniae strains isolated from liver abscesses. / Yeh, Kuo Ming; Chiu, Sheng Kung; Lin, Chii Lan; Huang, Li Yueh; Tsai, Yu Kuo; Chang, Jen Chang; Lin, Jung Chung; Chang, Feng Yee; Siu, Leung Kei.

In: Gut Pathogens, Vol. 8, No. 1, 4, 13.02.2016.

Research output: Contribution to journalArticle

Yeh, Kuo Ming ; Chiu, Sheng Kung ; Lin, Chii Lan ; Huang, Li Yueh ; Tsai, Yu Kuo ; Chang, Jen Chang ; Lin, Jung Chung ; Chang, Feng Yee ; Siu, Leung Kei. / Surface antigens contribute differently to the pathophysiological features in serotype K1 and K2 Klebsiella pneumoniae strains isolated from liver abscesses. In: Gut Pathogens. 2016 ; Vol. 8, No. 1.
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AU - Huang, Li Yueh

AU - Tsai, Yu Kuo

AU - Chang, Jen Chang

AU - Lin, Jung Chung

AU - Chang, Feng Yee

AU - Siu, Leung Kei

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N2 - Background: The virulence role of surface antigens in a single serotype of Klebsiella pneumoniae strain have been studied, but little is known about whether their contribution will vary with serotype. Method: To investigate the role of K and O antigen in hyper-virulent strains, we constructed O and K antigen deficient mutants from serotype K1 STL43 and K2 TSGH strains from patients with liver abscess, and characterized their virulence in according to the abscess formation and resistance to neutrophil phagocytosis, serum, and bacterial clearance in liver. Results: Both of K1 and K2-antigen mutants lost their wildtype resistance to neutrophil phagocytosis and hepatic clearance, and failed to cause abscess formation. K2-antigen mutant became serum susceptible while K1-antigen mutant maintained its resistance to serum killing. The amount of glucuronic acid, indicating the amount of capsular polysaccharide (CPS, K antigen), was inversed proportional to the rate of phagocytosis. O-antigen mutant of serotype K1 strains had significantly more amount of CPS, and more resistant to neutrophil phagocytosis than its wildtype counterpart. O-antigen mutants of serotype K1 and K2 strains lost their wildtype serum resistance, and kept resistant to neutrophil phagocytosis. While both mutants lacked the same O1 antigen, O-antigen mutant of serotype K1 became susceptible to liver clearance and cause mild abscess formation, but its serotype K2 counterpart maintained these wildtype virulence. Conclusion: We conclude that the contribution of surface antigens to virulence of K. pneumoniae strains varies with serotypes.

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