Surface analysis of titanium biological modification with glow discharge

Yu Chi Chang, Sheng Wei Feng, Haw Ming Huang, Nai Chia Teng, Che Tong Lin, Hsi Kuei Lin, Peter D. Wang, Wei Jen Chang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

BACKGROUND: Glow discharge plasma (GDP) technology has been used to graft various proteins to the titanium surface, including albumin, type I collagen, but without fibronectin.

PURPOSE: The aim of this study was to evaluate and analyze the physical properties of fibronectin-grafted titanium surfaces after GDP treatment.

MATERIALS AND METHODS: Grade II titanium discs after cleaning and autoclaving were considered as original specimens, thus divided into four groups. The groups were different upon two treatments (GDP only and fibronectin grafting after GDP) and two storage temperature (4°C and 25°C). The implant surface morphology was characterized by scanning electron microscopy (SEM), roughness measurement, and wettability evaluation. The concentration relationship of fibronectin was by fluorescein isothiocyanate (FITC) labeling.

RESULTS: SEM images showed that regular planar texture revealed on the surface of GDP-treated group, and irregular-folding protein was found on the fibronectin-grafted discs. Fibronectin-grafted groups had higher hydrophilicity and greater surface roughness than GDP-treated specimens. The storage temperature did not make obvious difference on the surface topography, wettability, and roughness. The number of fibronectin dots on the titanium surface labeling by FITC had positive relationship with the concentration of fibronectin solution used.

CONCLUSIONS: Biologically modified titanium surface is more hydrophilic and rougher than GDP-treated ones. GDP treatment combined with fibronectin grafting increased the surface hydrophilicity and surface roughness of titanium discs, which may attribute to the affinity of cell adhesion, migration, proliferation, and differentiation.

Original languageEnglish
Pages (from-to)469-475
Number of pages7
JournalClinical Implant Dentistry and Related Research
Volume17
Issue number3
DOIs
Publication statusPublished - Jun 1 2015

Fingerprint

Titanium
Fibronectins
Wettability
Fluorescein
Hydrophobic and Hydrophilic Interactions
Electron Scanning Microscopy
Temperature
Protein Folding
Collagen Type I
Cell Adhesion
Cell Movement
Albumins
Technology
Transplants

Keywords

  • fibronectin
  • glow discharge
  • implant design
  • surface properties
  • titanium

ASJC Scopus subject areas

  • Oral Surgery
  • Dentistry(all)

Cite this

Surface analysis of titanium biological modification with glow discharge. / Chang, Yu Chi; Feng, Sheng Wei; Huang, Haw Ming; Teng, Nai Chia; Lin, Che Tong; Lin, Hsi Kuei; Wang, Peter D.; Chang, Wei Jen.

In: Clinical Implant Dentistry and Related Research, Vol. 17, No. 3, 01.06.2015, p. 469-475.

Research output: Contribution to journalArticle

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AU - Wang, Peter D.

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N2 - BACKGROUND: Glow discharge plasma (GDP) technology has been used to graft various proteins to the titanium surface, including albumin, type I collagen, but without fibronectin.PURPOSE: The aim of this study was to evaluate and analyze the physical properties of fibronectin-grafted titanium surfaces after GDP treatment.MATERIALS AND METHODS: Grade II titanium discs after cleaning and autoclaving were considered as original specimens, thus divided into four groups. The groups were different upon two treatments (GDP only and fibronectin grafting after GDP) and two storage temperature (4°C and 25°C). The implant surface morphology was characterized by scanning electron microscopy (SEM), roughness measurement, and wettability evaluation. The concentration relationship of fibronectin was by fluorescein isothiocyanate (FITC) labeling.RESULTS: SEM images showed that regular planar texture revealed on the surface of GDP-treated group, and irregular-folding protein was found on the fibronectin-grafted discs. Fibronectin-grafted groups had higher hydrophilicity and greater surface roughness than GDP-treated specimens. The storage temperature did not make obvious difference on the surface topography, wettability, and roughness. The number of fibronectin dots on the titanium surface labeling by FITC had positive relationship with the concentration of fibronectin solution used.CONCLUSIONS: Biologically modified titanium surface is more hydrophilic and rougher than GDP-treated ones. GDP treatment combined with fibronectin grafting increased the surface hydrophilicity and surface roughness of titanium discs, which may attribute to the affinity of cell adhesion, migration, proliferation, and differentiation.

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