Suppressive effects of methyl methacrylate on the mutagenicity and DNA adduct formation induced by 1-nitropyrene and benzo[a]pyrene

Lin Shih Shen Chou, Shuang Miao Chao, Shuh Shyuan Bian, Shur Hueih Cherng, Ming Yung Chou, Huei Lee

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Abstract

Methyl methacrylate (MMA) is widely used as a cement in dentistry, orthopaedic surgery and ophthalmology. Studies based on short-term genotoxicity tests have produced conflicting results in the last two decades. In the present study, the effects of MMA on the mutagenicity of 1-nitropyrene (1-NP) and benzo[a]pyrene (B[a]P) were evaluated with the Salmonella typhimurium TA98 strain in the absence and presence of S9 mix. The direct-acting mutagenicity of 1-NP was markedly decreased by MMA in a dose-dependent manner. However, a low inhibitory effect of MMA on the metabolic-acting mutagenicity of B[a]P was observed. MMA did not show mutagenicity within the concentrations of 4.7-37.6 μM either with or without S9 mix. The inhibitory effect of MMA was not due to its cytotoxicity because very low and/or no cytotoxicity of MMA to S.typhimurium TA98 was observed. To confirm the antimutagenicity of MMA against 1-NP and B[a]P, a 32P-postlabelling method was used to determine whether MMA modified DNA adduct formation produced by both compounds in calf thymus DNA. MMA inhibits the formation of 1-NP- and B[a]P-DNA. adducts in a dose-dependent manner. The DNA adduct of 1-NP reduced by MMA was greater than that of B[a]P. Thus, we suggested that MMA was possibly acting as an inhibitor of chemical carcinogenesis.

Original languageEnglish
Pages (from-to)177-181
Number of pages5
JournalMutagenesis
Volume11
Issue number2
Publication statusPublished - Mar 1996
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics
  • Health, Toxicology and Mutagenesis
  • Genetics(clinical)
  • Toxicology

Cite this

Chou, L. S. S., Chao, S. M., Bian, S. S., Cherng, S. H., Chou, M. Y., & Lee, H. (1996). Suppressive effects of methyl methacrylate on the mutagenicity and DNA adduct formation induced by 1-nitropyrene and benzo[a]pyrene. Mutagenesis, 11(2), 177-181.