Suppressive effects of 3-O-methylquercetin on ovalbumin-induced airway hyperresponsiveness

Wun-Chang Ko, Chwen Ming Shih, Mei Chun Chen, Ya Hsin Lai, Jun Hao Chen, Chien Ming Chen, Chun Nan Lin

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Rhamnus nakaharai Hayata (Rhamnaceae) has been used as a folk medicine in Taiwan for treating constipation, inflammation, tumors, and asthma. 3-O-Methylquercetin (3-MQ), a main constituent of the plant, has been reported to inhibit total cAMP-and cGMP-phosphodiesterase (PDE) of guinea pig trachealis at low concentrations. 3-MQ has been also reported to more selectively inhibit PDE3 than PDE4 with a low Km value. Therefore we were interested in investigating its suppressive effects on ovalbumin (OVA)-induced airway hyperresponsiveness in vivo and in vitro. 3-MQ (3-30 μmol/kg, i.p.) significantly suppressed the enhanced pause (Penh) value induced by aerosolized methacholine (50 mg/mL) in sensitized mice after secondary allergen challenge. 3-MQ (3-30 μmol/kg, i.p.) also significantly suppressed total inflammatory cells, macrophages, neutrophils, and eosinophils, but not lymphocytes. In addition, 3-MQ (3 μmol/kg, i.p.) significantly decreased the secretion of TNF-α, and at the highest dose (30 μmol/kg, i.p.) even decreased the secretions of IL-4, IL-5, and TNF-α. 3-MQ (1-10 μM) as well as Ro 20-1724 (3-30 μM), a selective PDE4 inhibitor, significantly attenuated OVA (100 μg/ mL)-induced contractions. 3-MQ (30 μM) as well as milrinone (1 -10 μM), a selective PDE3 inhibitor, significantly enhanced baseline contractions in isolated guinea pig left and right atria. However, neither 3-MQ nor milrinone significantly affected baseline beating rate in the right atria. 3-MQ (3-30 μmol/kg, i.p.) did not significantly affect systolic pressure in conscious mice. In conclusion, 3-MQ has both anti-inflammatory and bronchodilating effects, and has the potential for use in the treatment of asthma at a dose without affecting blood pressure.

Original languageEnglish
Pages (from-to)1123-1127
Number of pages5
JournalPlanta Medica
Volume70
Issue number12
DOIs
Publication statusPublished - Dec 2004

Fingerprint

ovalbumin
Ovalbumin
asthma
guinea pigs
secretion
Rhamnus
Rhamnaceae
interleukin-5
constipation
mice
interleukin-4
eosinophils
dosage
traditional medicine
allergens
blood pressure
neutrophils
Taiwan
macrophages
lymphocytes

Keywords

  • 3-O-Methylquercetin
  • Asthma
  • Cytokines
  • Inflammatory cells
  • Ovalbumin-sensitized mice
  • Rhamnaceae
  • Rhamnus nakaharai

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Ko, W-C., Shih, C. M., Chen, M. C., Lai, Y. H., Chen, J. H., Chen, C. M., & Lin, C. N. (2004). Suppressive effects of 3-O-methylquercetin on ovalbumin-induced airway hyperresponsiveness. Planta Medica, 70(12), 1123-1127. https://doi.org/10.1055/s-2004-835838

Suppressive effects of 3-O-methylquercetin on ovalbumin-induced airway hyperresponsiveness. / Ko, Wun-Chang; Shih, Chwen Ming; Chen, Mei Chun; Lai, Ya Hsin; Chen, Jun Hao; Chen, Chien Ming; Lin, Chun Nan.

In: Planta Medica, Vol. 70, No. 12, 12.2004, p. 1123-1127.

Research output: Contribution to journalArticle

Ko, W-C, Shih, CM, Chen, MC, Lai, YH, Chen, JH, Chen, CM & Lin, CN 2004, 'Suppressive effects of 3-O-methylquercetin on ovalbumin-induced airway hyperresponsiveness', Planta Medica, vol. 70, no. 12, pp. 1123-1127. https://doi.org/10.1055/s-2004-835838
Ko, Wun-Chang ; Shih, Chwen Ming ; Chen, Mei Chun ; Lai, Ya Hsin ; Chen, Jun Hao ; Chen, Chien Ming ; Lin, Chun Nan. / Suppressive effects of 3-O-methylquercetin on ovalbumin-induced airway hyperresponsiveness. In: Planta Medica. 2004 ; Vol. 70, No. 12. pp. 1123-1127.
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