Suppression of the SOX2 neural effector gene by PRDM1 promotes human germ cell fate in embryonic stem cells

I. Ying Lin, Feng Lan Chiu, Chen Hsiang Yeang, Hsin Fu Chen, Ching Yu Chuang, Shii Yi Yang, Pei Shan Hou, Nardnisa Sintupisut, Hong Nerng Ho, Hung Chih Kuo, Kuo I. Lin

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The mechanisms of transcriptional regulation underlying human primordial germ cell (PGC) differentiation are largely unknown. The transcriptional repressor Prdm1/Blimp-1 is known to play a critical role in controlling germ cell specification in mice. Here, we show that PRDM1 is expressed in developing human gonads and contributes to the determination of germline versus neural fate in early development. We show that knockdown of PRDM1 in human embryonic stem cells (hESCs) impairs germline potential and upregulates neural genes. Conversely, ectopic expression of PRDM1 in hESCs promotes the generation of cells that exhibit phenotypic and transcriptomic features of early PGCs. Furthermore, PRDM1 suppresses transcription of SOX2. Overexpression of SOX2 in hESCs under conditions favoring germline differentiation skews cell fate from the germline to the neural lineage. Collectively, our results demonstrate that PRDM1 serves as a molecular switch to modulate the divergence of neural or germline fates through repression of SOX2 during human development.

Original languageEnglish
Pages (from-to)189-204
Number of pages16
JournalStem Cell Reports
Volume2
Issue number2
DOIs
Publication statusPublished - Feb 11 2014
Externally publishedYes

Fingerprint

Wnt3A Protein
SOXB1 Transcription Factors
Bone Morphogenetic Protein 4
Repressor Proteins
Stem cells
Genes
Cells
Airships
Transcription
Switches
Specifications

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Lin, I. Y., Chiu, F. L., Yeang, C. H., Chen, H. F., Chuang, C. Y., Yang, S. Y., ... Lin, K. I. (2014). Suppression of the SOX2 neural effector gene by PRDM1 promotes human germ cell fate in embryonic stem cells. Stem Cell Reports, 2(2), 189-204. https://doi.org/10.1016/j.stemcr.2013.12.009

Suppression of the SOX2 neural effector gene by PRDM1 promotes human germ cell fate in embryonic stem cells. / Lin, I. Ying; Chiu, Feng Lan; Yeang, Chen Hsiang; Chen, Hsin Fu; Chuang, Ching Yu; Yang, Shii Yi; Hou, Pei Shan; Sintupisut, Nardnisa; Ho, Hong Nerng; Kuo, Hung Chih; Lin, Kuo I.

In: Stem Cell Reports, Vol. 2, No. 2, 11.02.2014, p. 189-204.

Research output: Contribution to journalArticle

Lin, IY, Chiu, FL, Yeang, CH, Chen, HF, Chuang, CY, Yang, SY, Hou, PS, Sintupisut, N, Ho, HN, Kuo, HC & Lin, KI 2014, 'Suppression of the SOX2 neural effector gene by PRDM1 promotes human germ cell fate in embryonic stem cells', Stem Cell Reports, vol. 2, no. 2, pp. 189-204. https://doi.org/10.1016/j.stemcr.2013.12.009
Lin, I. Ying ; Chiu, Feng Lan ; Yeang, Chen Hsiang ; Chen, Hsin Fu ; Chuang, Ching Yu ; Yang, Shii Yi ; Hou, Pei Shan ; Sintupisut, Nardnisa ; Ho, Hong Nerng ; Kuo, Hung Chih ; Lin, Kuo I. / Suppression of the SOX2 neural effector gene by PRDM1 promotes human germ cell fate in embryonic stem cells. In: Stem Cell Reports. 2014 ; Vol. 2, No. 2. pp. 189-204.
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