Suppression of lipopolysaccharide-induced of inducible nitric oxide synthase and cyclooxygenase-2 by Sanguis Draconis, a dragon's blood resin, in RAW 264.7 cells

Cheuk Sing Choy, Chien Ming Hu, Wen Ta Chiu, Carlos Lam, Yih Ting, Shin Han Tsai, Tzu Chien Wang

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Sanguis Draconis (SD) is a kind of dragon's blood resin that is obtained from Daemomorops draco (Palmae). It is used in traditional medicine and has shown anti-inflammatory activity in some diseases. In this study, we examined the effects of Sanguis Dranonis ethanol extract (SDEE) on LPS-induced inflammation using RAW 264.7 cells. Our data indicated that SDEE inhibits LPS-stimulated NO, PGE2, IL-1β and TNF-α release, and iNOS and COX-2 expression. Furthermore, SDEE suppressed the LPS-induced p65 expression of NF-κB, which was associated with the inhibition of IκB-α degradation. We also found that the expression of HO-1 was significantly increased in RAW 264.7 cells by SDEE. These results suggest among possibilities of anti-inflammation that SDEE inhibits the production of NO and PGE2 by the down-regulation of iNOS and COX-2 gene expression via the suppression of NF-κB (p65) activation. SDEE can induce HO-1 over-expression in macrophage cells, which indicates that it may possess antioxidant properties. This result means that SEDD its anti-inflammatory effects in macrophages may be through a novel mechanism that involves the action of HO-1. Thus, SD could provide a potential therapeutic approach for inflammation-associated disorders.

Original languageEnglish
Pages (from-to)455-462
Number of pages8
JournalJournal of Ethnopharmacology
Volume115
Issue number3
DOIs
Publication statusPublished - Feb 12 2007

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Keywords

  • Cyclooxygenase-2
  • Inducible nitric oxide synthase
  • Lipopolysaccharide
  • Nuclear factor-κB
  • Sanguis Draconis

ASJC Scopus subject areas

  • Pharmacology

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