Suppression of inflammation-associated factors by indole-3-carbinol in mice fed high-fat diets and in isolated, co-cultured macrophages and adipocytes

H. P. Chang, M. L. Wang, C. Y. Hsu, M. E. Liu, M. H. Chan, Yue-Hwa Chen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Aims:This study investigated the effects of indole-3-carbinol (I3C), a compound from cruciferous vegetables, on various parameters related to obesity, in particular, the parameters of infiltration by macrophages and of inflammatory cytokines expressed during the co-culture of adipocytes and macrophages.Methods:Male C57BL/6 mice were fed with a control diet (C group), high-fat diet (HF group) and HF5 mg kg -1 I3C (HFI group). The I3C was intraperitoneally injected (HFI group) for 12 weeks. Epididymal adipose tissue (AT) was collected and stained for F4/80, a marker of macrophages.Results:The immunohistochemical staining for F4/80 indicated a greater presence of macrophages in the HF group than in AT from the control and HFI groups. Furthermore, I3C treatment, in an in vitro cell culture system, decreased expression of inducible nitric oxide synthase (iNOS), decreased nitrite content and enhanced expression of peroxisome proliferator-activated receptor (PPAR-γ). Moreover, in vitro cell culture studies revealed that I3C inhibited intracellular lipid accumulation in hypertrophied adipocytes. In macrophage and primary adipocyte co-culture, I3C inhibited expression of interleukin-6 (IL-6).Conclusions:In vivo treatment with I3C reduced the infiltration of macrophages in AT, and in vitro addition of I3C to co-cultured macrophages and adipocytes reduced nitrite production and IL-6 expression. With cultures of adipocytes only, I3C inhibited accumulation of intracellular lipid, either by disrupting differentiation, or by directly inhibiting triglyceride synthesis.

Original languageEnglish
Pages (from-to)1530-1538
Number of pages9
JournalInternational Journal of Obesity
Volume35
Issue number12
DOIs
Publication statusPublished - Dec 2011

Fingerprint

High Fat Diet
Adipocytes
Macrophages
Inflammation
Adipose Tissue
Nitrites
Coculture Techniques
Interleukin-6
Cell Culture Techniques
Lipids
indole-3-carbinol
Peroxisome Proliferator-Activated Receptors
Nitric Oxide Synthase Type II
Inbred C57BL Mouse
Vegetables
Triglycerides
Obesity
Staining and Labeling
Cytokines
Diet

Keywords

  • adipocytes
  • Indole-3-carbinol
  • inflammation
  • macrophages

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Endocrinology, Diabetes and Metabolism

Cite this

Suppression of inflammation-associated factors by indole-3-carbinol in mice fed high-fat diets and in isolated, co-cultured macrophages and adipocytes. / Chang, H. P.; Wang, M. L.; Hsu, C. Y.; Liu, M. E.; Chan, M. H.; Chen, Yue-Hwa.

In: International Journal of Obesity, Vol. 35, No. 12, 12.2011, p. 1530-1538.

Research output: Contribution to journalArticle

@article{16f5fae9732c4129a4f503637c19ca8f,
title = "Suppression of inflammation-associated factors by indole-3-carbinol in mice fed high-fat diets and in isolated, co-cultured macrophages and adipocytes",
abstract = "Aims:This study investigated the effects of indole-3-carbinol (I3C), a compound from cruciferous vegetables, on various parameters related to obesity, in particular, the parameters of infiltration by macrophages and of inflammatory cytokines expressed during the co-culture of adipocytes and macrophages.Methods:Male C57BL/6 mice were fed with a control diet (C group), high-fat diet (HF group) and HF5 mg kg -1 I3C (HFI group). The I3C was intraperitoneally injected (HFI group) for 12 weeks. Epididymal adipose tissue (AT) was collected and stained for F4/80, a marker of macrophages.Results:The immunohistochemical staining for F4/80 indicated a greater presence of macrophages in the HF group than in AT from the control and HFI groups. Furthermore, I3C treatment, in an in vitro cell culture system, decreased expression of inducible nitric oxide synthase (iNOS), decreased nitrite content and enhanced expression of peroxisome proliferator-activated receptor (PPAR-γ). Moreover, in vitro cell culture studies revealed that I3C inhibited intracellular lipid accumulation in hypertrophied adipocytes. In macrophage and primary adipocyte co-culture, I3C inhibited expression of interleukin-6 (IL-6).Conclusions:In vivo treatment with I3C reduced the infiltration of macrophages in AT, and in vitro addition of I3C to co-cultured macrophages and adipocytes reduced nitrite production and IL-6 expression. With cultures of adipocytes only, I3C inhibited accumulation of intracellular lipid, either by disrupting differentiation, or by directly inhibiting triglyceride synthesis.",
keywords = "adipocytes, Indole-3-carbinol, inflammation, macrophages",
author = "Chang, {H. P.} and Wang, {M. L.} and Hsu, {C. Y.} and Liu, {M. E.} and Chan, {M. H.} and Yue-Hwa Chen",
year = "2011",
month = "12",
doi = "10.1038/ijo.2011.12",
language = "English",
volume = "35",
pages = "1530--1538",
journal = "International Journal of Obesity",
issn = "0307-0565",
publisher = "Nature Publishing Group",
number = "12",

}

TY - JOUR

T1 - Suppression of inflammation-associated factors by indole-3-carbinol in mice fed high-fat diets and in isolated, co-cultured macrophages and adipocytes

AU - Chang, H. P.

AU - Wang, M. L.

AU - Hsu, C. Y.

AU - Liu, M. E.

AU - Chan, M. H.

AU - Chen, Yue-Hwa

PY - 2011/12

Y1 - 2011/12

N2 - Aims:This study investigated the effects of indole-3-carbinol (I3C), a compound from cruciferous vegetables, on various parameters related to obesity, in particular, the parameters of infiltration by macrophages and of inflammatory cytokines expressed during the co-culture of adipocytes and macrophages.Methods:Male C57BL/6 mice were fed with a control diet (C group), high-fat diet (HF group) and HF5 mg kg -1 I3C (HFI group). The I3C was intraperitoneally injected (HFI group) for 12 weeks. Epididymal adipose tissue (AT) was collected and stained for F4/80, a marker of macrophages.Results:The immunohistochemical staining for F4/80 indicated a greater presence of macrophages in the HF group than in AT from the control and HFI groups. Furthermore, I3C treatment, in an in vitro cell culture system, decreased expression of inducible nitric oxide synthase (iNOS), decreased nitrite content and enhanced expression of peroxisome proliferator-activated receptor (PPAR-γ). Moreover, in vitro cell culture studies revealed that I3C inhibited intracellular lipid accumulation in hypertrophied adipocytes. In macrophage and primary adipocyte co-culture, I3C inhibited expression of interleukin-6 (IL-6).Conclusions:In vivo treatment with I3C reduced the infiltration of macrophages in AT, and in vitro addition of I3C to co-cultured macrophages and adipocytes reduced nitrite production and IL-6 expression. With cultures of adipocytes only, I3C inhibited accumulation of intracellular lipid, either by disrupting differentiation, or by directly inhibiting triglyceride synthesis.

AB - Aims:This study investigated the effects of indole-3-carbinol (I3C), a compound from cruciferous vegetables, on various parameters related to obesity, in particular, the parameters of infiltration by macrophages and of inflammatory cytokines expressed during the co-culture of adipocytes and macrophages.Methods:Male C57BL/6 mice were fed with a control diet (C group), high-fat diet (HF group) and HF5 mg kg -1 I3C (HFI group). The I3C was intraperitoneally injected (HFI group) for 12 weeks. Epididymal adipose tissue (AT) was collected and stained for F4/80, a marker of macrophages.Results:The immunohistochemical staining for F4/80 indicated a greater presence of macrophages in the HF group than in AT from the control and HFI groups. Furthermore, I3C treatment, in an in vitro cell culture system, decreased expression of inducible nitric oxide synthase (iNOS), decreased nitrite content and enhanced expression of peroxisome proliferator-activated receptor (PPAR-γ). Moreover, in vitro cell culture studies revealed that I3C inhibited intracellular lipid accumulation in hypertrophied adipocytes. In macrophage and primary adipocyte co-culture, I3C inhibited expression of interleukin-6 (IL-6).Conclusions:In vivo treatment with I3C reduced the infiltration of macrophages in AT, and in vitro addition of I3C to co-cultured macrophages and adipocytes reduced nitrite production and IL-6 expression. With cultures of adipocytes only, I3C inhibited accumulation of intracellular lipid, either by disrupting differentiation, or by directly inhibiting triglyceride synthesis.

KW - adipocytes

KW - Indole-3-carbinol

KW - inflammation

KW - macrophages

UR - http://www.scopus.com/inward/record.url?scp=84856438998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856438998&partnerID=8YFLogxK

U2 - 10.1038/ijo.2011.12

DO - 10.1038/ijo.2011.12

M3 - Article

C2 - 21343904

AN - SCOPUS:84856438998

VL - 35

SP - 1530

EP - 1538

JO - International Journal of Obesity

JF - International Journal of Obesity

SN - 0307-0565

IS - 12

ER -