Suppression of inducible nitric oxide production by indole and isothiocyanate derivatives from Brassica plants in stimulated macrophages

Yue Hwa Chen, Huey Jing Dai, Hsiao Pei Chang

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32 Citations (Scopus)


In this study, the effects of bioactive compounds derived from vegetables of the Brassica genus (Brassicaceae) including 2-phenylethyl isothiocyanate (PEITC), indole-3-carbinol (13C), and indolo[3,2-b]carbazole (ICZ), on the inhibition of NO production in RAW 264.7 cells were explored. The results indicated that PEITC and 13C inhibited lipopolysaccharide (LPS)- and interferon-γ (IFN-γ)-induced NO production in RAW 264.7 cells, and this inhibition was in accordance with lowering the expression of iNOS protein and mRNA. On the contrary, ICZ, a derivative of 13C, had no significant effect on the stimulated NO production. In conclusion, the Brassica plants derivatives, PEITC and, to a lesser extent, 13C inhibit the LPS/IFN-γ-induced NO production by lowering iNOS protein and mRNA expression in RAW 264.7 cells, in which the PEITC had a more potent inhibitory effect. Nevertheless, ICZ exhibits no inhibitory effect on the activated NO production (Indole-3-carbinol = indole-3-methanol).

Original languageEnglish
Pages (from-to)696-700
Number of pages5
JournalPlanta Medica
Issue number8
Publication statusPublished - Aug 1 2003



  • Brassicaceae
  • Indole
  • Inducible nitric oxide synthase
  • Isothiocyanate
  • Nitric oxide
  • RAW 264.7 macrophages

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

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