Suppression of inducible nitric oxide production by indole and isothiocyanate derivatives from Brassica plants in stimulated macrophages

Yue Hwa Chen, Huey Jing Dai, Hsiao Pei Chang

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In this study, the effects of bioactive compounds derived from vegetables of the Brassica genus (Brassicaceae) including 2-phenylethyl isothiocyanate (PEITC), indole-3-carbinol (13C), and indolo[3,2-b]carbazole (ICZ), on the inhibition of NO production in RAW 264.7 cells were explored. The results indicated that PEITC and 13C inhibited lipopolysaccharide (LPS)- and interferon-γ (IFN-γ)-induced NO production in RAW 264.7 cells, and this inhibition was in accordance with lowering the expression of iNOS protein and mRNA. On the contrary, ICZ, a derivative of 13C, had no significant effect on the stimulated NO production. In conclusion, the Brassica plants derivatives, PEITC and, to a lesser extent, 13C inhibit the LPS/IFN-γ-induced NO production by lowering iNOS protein and mRNA expression in RAW 264.7 cells, in which the PEITC had a more potent inhibitory effect. Nevertheless, ICZ exhibits no inhibitory effect on the activated NO production (Indole-3-carbinol = indole-3-methanol).

Original languageEnglish
Pages (from-to)696-700
Number of pages5
JournalPlanta Medica
Volume69
Issue number8
DOIs
Publication statusPublished - Aug 1 2003

Fingerprint

Macrophages
Brassica
isothiocyanates
indoles
nitric oxide
Nitric Oxide
macrophages
chemical derivatives
Derivatives
interferons
Interferons
lipopolysaccharides
indole-3-methanol
Lipopolysaccharides
carbazoles
Brassicaceae
Messenger RNA
cells
Vegetables
Proteins

Keywords

  • Brassicaceae
  • Indole
  • Inducible nitric oxide synthase
  • Isothiocyanate
  • Nitric oxide
  • RAW 264.7 macrophages

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Suppression of inducible nitric oxide production by indole and isothiocyanate derivatives from Brassica plants in stimulated macrophages. / Chen, Yue Hwa; Dai, Huey Jing; Chang, Hsiao Pei.

In: Planta Medica, Vol. 69, No. 8, 01.08.2003, p. 696-700.

Research output: Contribution to journalArticle

@article{a2175b53176f4e57b5af4a4faade4868,
title = "Suppression of inducible nitric oxide production by indole and isothiocyanate derivatives from Brassica plants in stimulated macrophages",
abstract = "In this study, the effects of bioactive compounds derived from vegetables of the Brassica genus (Brassicaceae) including 2-phenylethyl isothiocyanate (PEITC), indole-3-carbinol (13C), and indolo[3,2-b]carbazole (ICZ), on the inhibition of NO production in RAW 264.7 cells were explored. The results indicated that PEITC and 13C inhibited lipopolysaccharide (LPS)- and interferon-γ (IFN-γ)-induced NO production in RAW 264.7 cells, and this inhibition was in accordance with lowering the expression of iNOS protein and mRNA. On the contrary, ICZ, a derivative of 13C, had no significant effect on the stimulated NO production. In conclusion, the Brassica plants derivatives, PEITC and, to a lesser extent, 13C inhibit the LPS/IFN-γ-induced NO production by lowering iNOS protein and mRNA expression in RAW 264.7 cells, in which the PEITC had a more potent inhibitory effect. Nevertheless, ICZ exhibits no inhibitory effect on the activated NO production (Indole-3-carbinol = indole-3-methanol).",
keywords = "Brassicaceae, Indole, Inducible nitric oxide synthase, Isothiocyanate, Nitric oxide, RAW 264.7 macrophages",
author = "Chen, {Yue Hwa} and Dai, {Huey Jing} and Chang, {Hsiao Pei}",
year = "2003",
month = "8",
day = "1",
doi = "10.1055/s-2003-42790",
language = "English",
volume = "69",
pages = "696--700",
journal = "Planta Medica",
issn = "0032-0943",
publisher = "Georg Thieme Verlag",
number = "8",

}

TY - JOUR

T1 - Suppression of inducible nitric oxide production by indole and isothiocyanate derivatives from Brassica plants in stimulated macrophages

AU - Chen, Yue Hwa

AU - Dai, Huey Jing

AU - Chang, Hsiao Pei

PY - 2003/8/1

Y1 - 2003/8/1

N2 - In this study, the effects of bioactive compounds derived from vegetables of the Brassica genus (Brassicaceae) including 2-phenylethyl isothiocyanate (PEITC), indole-3-carbinol (13C), and indolo[3,2-b]carbazole (ICZ), on the inhibition of NO production in RAW 264.7 cells were explored. The results indicated that PEITC and 13C inhibited lipopolysaccharide (LPS)- and interferon-γ (IFN-γ)-induced NO production in RAW 264.7 cells, and this inhibition was in accordance with lowering the expression of iNOS protein and mRNA. On the contrary, ICZ, a derivative of 13C, had no significant effect on the stimulated NO production. In conclusion, the Brassica plants derivatives, PEITC and, to a lesser extent, 13C inhibit the LPS/IFN-γ-induced NO production by lowering iNOS protein and mRNA expression in RAW 264.7 cells, in which the PEITC had a more potent inhibitory effect. Nevertheless, ICZ exhibits no inhibitory effect on the activated NO production (Indole-3-carbinol = indole-3-methanol).

AB - In this study, the effects of bioactive compounds derived from vegetables of the Brassica genus (Brassicaceae) including 2-phenylethyl isothiocyanate (PEITC), indole-3-carbinol (13C), and indolo[3,2-b]carbazole (ICZ), on the inhibition of NO production in RAW 264.7 cells were explored. The results indicated that PEITC and 13C inhibited lipopolysaccharide (LPS)- and interferon-γ (IFN-γ)-induced NO production in RAW 264.7 cells, and this inhibition was in accordance with lowering the expression of iNOS protein and mRNA. On the contrary, ICZ, a derivative of 13C, had no significant effect on the stimulated NO production. In conclusion, the Brassica plants derivatives, PEITC and, to a lesser extent, 13C inhibit the LPS/IFN-γ-induced NO production by lowering iNOS protein and mRNA expression in RAW 264.7 cells, in which the PEITC had a more potent inhibitory effect. Nevertheless, ICZ exhibits no inhibitory effect on the activated NO production (Indole-3-carbinol = indole-3-methanol).

KW - Brassicaceae

KW - Indole

KW - Inducible nitric oxide synthase

KW - Isothiocyanate

KW - Nitric oxide

KW - RAW 264.7 macrophages

UR - http://www.scopus.com/inward/record.url?scp=0141816815&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141816815&partnerID=8YFLogxK

U2 - 10.1055/s-2003-42790

DO - 10.1055/s-2003-42790

M3 - Article

C2 - 14531017

AN - SCOPUS:0141816815

VL - 69

SP - 696

EP - 700

JO - Planta Medica

JF - Planta Medica

SN - 0032-0943

IS - 8

ER -