Suppression of extracellular signals and cell proliferation by the black tea polyphenol, theaflavin-3,3'-digallate

Yu Chih Liang, Yen Chou Chen, Yu Li Lin, Shoei Yn Lin-Shiau, Chi Tang Ho, Jen Kun Lin

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Previous studies in our laboratory have shown that the major green tea polyphenol, (-)-epigallocatechin-3-gallate (EGCG), suppressed autophosphorylation of epidermal growth factor (EGF) receptor induced by EGF in human A431 epidermoid carcinoma cells. In this study, we examined the inhibitory effects of black tea polyphenols, including theaflavin (TF-1), a mixture (TF-2) of theaflavin-3-gallate (TF-2a) and theaflavin-3'-gallate (TF-2b), theaflavin-3,3'-digallate (TF-3) and the thearubigin fraction on the autophosphorylation of the EGF and PDGF receptors in A431 cells and mouse NIH3T3 fibroblast cells, respectively. First, we examined the effects of these polyphenols on the proliferation of A431 and NIH3T3 cells. Both EGCG and TF-3 strongly inhibited the proliferation of A431 and NIH3T3 cells more than the other theaflavins did. In cultured cells with pre-treatment of tea polyphenol, TF-3 was stronger than EGCG on the reduction of EGF receptor and PDGF receptor autophosphorylation induced by EGF and PDGF, respectively. Other theaflavins slightly reduced the autophosphorylation of the EGF and PDGF receptors; furthermore, TF-3 could reduce autophosphorylation of the EGF receptor (or PDGF receptor) even with co-treatment with EGF (or PDGF) and TF-3, but EGCG was inactive under these conditions. In addition, TF-3 was stronger than EGCG in blocking EGF binding to its receptor. These results suggest that not only the green tea polyphenol, EGCG, but also the black tea polyphenol, TF-3, have an antiproliferative activity on tumor cells, and the molecular mechanisms of antiproliferation may block the growth factor binding to its receptor and thus suppress mitogenic signal transduction.

Original languageEnglish
Pages (from-to)733-736
Number of pages4
JournalCarcinogenesis
Volume20
Issue number4
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Polyphenols
Tea
Cell Proliferation
Epidermal Growth Factor
Epidermal Growth Factor Receptor
Platelet-Derived Growth Factor Receptors
theaflavin-3,3'-digallate
epigallocatechin gallate
Squamous Cell Carcinoma
Cultured Cells
Signal Transduction
Intercellular Signaling Peptides and Proteins
Fibroblasts
theaflavin
Neoplasms

ASJC Scopus subject areas

  • Cancer Research

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Suppression of extracellular signals and cell proliferation by the black tea polyphenol, theaflavin-3,3'-digallate. / Liang, Yu Chih; Chen, Yen Chou; Lin, Yu Li; Lin-Shiau, Shoei Yn; Ho, Chi Tang; Lin, Jen Kun.

In: Carcinogenesis, Vol. 20, No. 4, 1999, p. 733-736.

Research output: Contribution to journalArticle

Liang, Yu Chih ; Chen, Yen Chou ; Lin, Yu Li ; Lin-Shiau, Shoei Yn ; Ho, Chi Tang ; Lin, Jen Kun. / Suppression of extracellular signals and cell proliferation by the black tea polyphenol, theaflavin-3,3'-digallate. In: Carcinogenesis. 1999 ; Vol. 20, No. 4. pp. 733-736.
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AU - Liang, Yu Chih

AU - Chen, Yen Chou

AU - Lin, Yu Li

AU - Lin-Shiau, Shoei Yn

AU - Ho, Chi Tang

AU - Lin, Jen Kun

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