Suppression of cell growth by heavy chain ferritin

Jia Hsin Guo, Shu Hui Juan, Steven D. Aust

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

While producing recombinant rat liver H and L chain ferritin homopolymers using the baculovirus expression system, we noticed that rat liver H chain ferritin, but not L chain ferritin, had a suppressive effect on the growth of Spodoptera frugiperda (Sf-21) cells. Suppression was observed immediately after infection with recombinant H chain ferritin baculovirus prepared from lysed infected cells. Immediate suppression was observed when purified with either recombinant H chain apoferritin or various holoferritins (loaded with 1970 ± 50 or 2520 ± 90 atoms of iron/ ferritin) indicating that suppression was not due to sequestration of iron required for cell growth. Suppression by H chain ferritin was also observed upon attempting to express the protein in Escherichia coli. Strategies for expression of recombinant rat liver H and L ferritin homopolymers in both prokaryotic and eukaryotic expression systems were developed.

Original languageEnglish
Pages (from-to)39-45
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume242
Issue number1
DOIs
Publication statusPublished - Jan 6 1998
Externally publishedYes

Fingerprint

Apoferritins
Cell growth
Ferritins
Growth
Liver
Rats
Homopolymerization
Baculoviridae
Iron
Sf9 Cells
Escherichia coli
Escherichia coli Proteins
Atoms

Keywords

  • Cell suppression
  • Iron
  • Recombinant ferritin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Suppression of cell growth by heavy chain ferritin. / Guo, Jia Hsin; Juan, Shu Hui; Aust, Steven D.

In: Biochemical and Biophysical Research Communications, Vol. 242, No. 1, 06.01.1998, p. 39-45.

Research output: Contribution to journalArticle

@article{4fde15fdc14046cc99452d2ec13bd6b5,
title = "Suppression of cell growth by heavy chain ferritin",
abstract = "While producing recombinant rat liver H and L chain ferritin homopolymers using the baculovirus expression system, we noticed that rat liver H chain ferritin, but not L chain ferritin, had a suppressive effect on the growth of Spodoptera frugiperda (Sf-21) cells. Suppression was observed immediately after infection with recombinant H chain ferritin baculovirus prepared from lysed infected cells. Immediate suppression was observed when purified with either recombinant H chain apoferritin or various holoferritins (loaded with 1970 ± 50 or 2520 ± 90 atoms of iron/ ferritin) indicating that suppression was not due to sequestration of iron required for cell growth. Suppression by H chain ferritin was also observed upon attempting to express the protein in Escherichia coli. Strategies for expression of recombinant rat liver H and L ferritin homopolymers in both prokaryotic and eukaryotic expression systems were developed.",
keywords = "Cell suppression, Iron, Recombinant ferritin",
author = "Guo, {Jia Hsin} and Juan, {Shu Hui} and Aust, {Steven D.}",
year = "1998",
month = "1",
day = "6",
doi = "10.1006/bbrc.1997.7910",
language = "English",
volume = "242",
pages = "39--45",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier B.V.",
number = "1",

}

TY - JOUR

T1 - Suppression of cell growth by heavy chain ferritin

AU - Guo, Jia Hsin

AU - Juan, Shu Hui

AU - Aust, Steven D.

PY - 1998/1/6

Y1 - 1998/1/6

N2 - While producing recombinant rat liver H and L chain ferritin homopolymers using the baculovirus expression system, we noticed that rat liver H chain ferritin, but not L chain ferritin, had a suppressive effect on the growth of Spodoptera frugiperda (Sf-21) cells. Suppression was observed immediately after infection with recombinant H chain ferritin baculovirus prepared from lysed infected cells. Immediate suppression was observed when purified with either recombinant H chain apoferritin or various holoferritins (loaded with 1970 ± 50 or 2520 ± 90 atoms of iron/ ferritin) indicating that suppression was not due to sequestration of iron required for cell growth. Suppression by H chain ferritin was also observed upon attempting to express the protein in Escherichia coli. Strategies for expression of recombinant rat liver H and L ferritin homopolymers in both prokaryotic and eukaryotic expression systems were developed.

AB - While producing recombinant rat liver H and L chain ferritin homopolymers using the baculovirus expression system, we noticed that rat liver H chain ferritin, but not L chain ferritin, had a suppressive effect on the growth of Spodoptera frugiperda (Sf-21) cells. Suppression was observed immediately after infection with recombinant H chain ferritin baculovirus prepared from lysed infected cells. Immediate suppression was observed when purified with either recombinant H chain apoferritin or various holoferritins (loaded with 1970 ± 50 or 2520 ± 90 atoms of iron/ ferritin) indicating that suppression was not due to sequestration of iron required for cell growth. Suppression by H chain ferritin was also observed upon attempting to express the protein in Escherichia coli. Strategies for expression of recombinant rat liver H and L ferritin homopolymers in both prokaryotic and eukaryotic expression systems were developed.

KW - Cell suppression

KW - Iron

KW - Recombinant ferritin

UR - http://www.scopus.com/inward/record.url?scp=0032488542&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032488542&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1997.7910

DO - 10.1006/bbrc.1997.7910

M3 - Article

C2 - 9439606

AN - SCOPUS:0032488542

VL - 242

SP - 39

EP - 45

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -