Suppression of cell growth by heavy chain ferritin

Jia Hsin Guo, Shu Hui Juan, Steven D. Aust

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11 Citations (Scopus)

Abstract

While producing recombinant rat liver H and L chain ferritin homopolymers using the baculovirus expression system, we noticed that rat liver H chain ferritin, but not L chain ferritin, had a suppressive effect on the growth of Spodoptera frugiperda (Sf-21) cells. Suppression was observed immediately after infection with recombinant H chain ferritin baculovirus prepared from lysed infected cells. Immediate suppression was observed when purified with either recombinant H chain apoferritin or various holoferritins (loaded with 1970 ± 50 or 2520 ± 90 atoms of iron/ ferritin) indicating that suppression was not due to sequestration of iron required for cell growth. Suppression by H chain ferritin was also observed upon attempting to express the protein in Escherichia coli. Strategies for expression of recombinant rat liver H and L ferritin homopolymers in both prokaryotic and eukaryotic expression systems were developed.

Original languageEnglish
Pages (from-to)39-45
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume242
Issue number1
DOIs
Publication statusPublished - Jan 6 1998
Externally publishedYes

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Keywords

  • Cell suppression
  • Iron
  • Recombinant ferritin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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