Supplemental dietary arginine reduces renal RAGE expression and oxidative damage in rats with streptozotocin-induced type 2 diabetes

Kuan Hsun Huang, Man H. Pai, Ching Hsiang Wu, Jun J. Liu, Sung Ling Yeh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background & aims: Arginine (Arg) was shown to have immunomodulatory effect and inhibits advanced glycation end product (AGE) formation in in vitro studies. This study investigated the effects of dietary Arg supplementation on renal receptor of AGE (RAGE) expressions and oxidative damage in diabetic rats. Methods: There were 1 normal control (NC) group and 2 diabetic groups in this study. Rats in the NC group were fed with a chow diet. One diabetic group (DM) was fed a common semipurified diet, while the other diabetic group received a diet in which part of the casein was replaced by Arg (DM-Arg) for 8 wk. Diabetes was induced by an intraperitoneal injection of nicotinamide followed by streptozotocin. Rats with blood glucose levels exceeding 180 mg/dL were considered diabetic. Blood samples were collected at the baseline and 8 wk. Kidneys of the animals were harvested at the end of the study for further analysis. Results: Plasma fructosamine contents were significantly higher in the diabetic groups than in the NC group. The DM group had higher fructosamine than the DM-Arg group. Kidney nitrotyrosine concentrations and nuclear factor-κB p65 protein expressions were significantly lower in the DM-Arg group than in the DM group. The result of immunohistochemical staining also showed that the expressions of RAGE in the kidneys were significantly lower in the DM-Arg group than in the DM group. Conclusions: These results suggest that dietary Arg supplementation may decrease renal RAGE expressions and oxidative damage in rats with type 2 diabetes.

Original languageEnglish
Journale-SPEN
Volume5
Issue number2
DOIs
Publication statusPublished - Apr 2010

Fingerprint

Streptozocin
Type 2 Diabetes Mellitus
Arginine
Kidney
Fructosamine
Dietary Supplements
Diet
Control Groups
Advanced Glycosylation End Products
Niacinamide
Advanced Glycosylation End Product-Specific Receptor
Caseins
Intraperitoneal Injections
Blood Glucose
Staining and Labeling

Keywords

  • Arginine
  • Fructosamine
  • Nitrotyrosine
  • RAGE expression
  • Type 2 diabetes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism

Cite this

@article{d9fae86032cf4cf8bdde7606d3272370,
title = "Supplemental dietary arginine reduces renal RAGE expression and oxidative damage in rats with streptozotocin-induced type 2 diabetes",
abstract = "Background & aims: Arginine (Arg) was shown to have immunomodulatory effect and inhibits advanced glycation end product (AGE) formation in in vitro studies. This study investigated the effects of dietary Arg supplementation on renal receptor of AGE (RAGE) expressions and oxidative damage in diabetic rats. Methods: There were 1 normal control (NC) group and 2 diabetic groups in this study. Rats in the NC group were fed with a chow diet. One diabetic group (DM) was fed a common semipurified diet, while the other diabetic group received a diet in which part of the casein was replaced by Arg (DM-Arg) for 8 wk. Diabetes was induced by an intraperitoneal injection of nicotinamide followed by streptozotocin. Rats with blood glucose levels exceeding 180 mg/dL were considered diabetic. Blood samples were collected at the baseline and 8 wk. Kidneys of the animals were harvested at the end of the study for further analysis. Results: Plasma fructosamine contents were significantly higher in the diabetic groups than in the NC group. The DM group had higher fructosamine than the DM-Arg group. Kidney nitrotyrosine concentrations and nuclear factor-κB p65 protein expressions were significantly lower in the DM-Arg group than in the DM group. The result of immunohistochemical staining also showed that the expressions of RAGE in the kidneys were significantly lower in the DM-Arg group than in the DM group. Conclusions: These results suggest that dietary Arg supplementation may decrease renal RAGE expressions and oxidative damage in rats with type 2 diabetes.",
keywords = "Arginine, Fructosamine, Nitrotyrosine, RAGE expression, Type 2 diabetes",
author = "Huang, {Kuan Hsun} and Pai, {Man H.} and Wu, {Ching Hsiang} and Liu, {Jun J.} and Yeh, {Sung Ling}",
year = "2010",
month = "4",
doi = "10.1016/j.eclnm.2010.02.001",
language = "English",
volume = "5",
journal = "e-SPEN",
issn = "2212-8263",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Supplemental dietary arginine reduces renal RAGE expression and oxidative damage in rats with streptozotocin-induced type 2 diabetes

AU - Huang, Kuan Hsun

AU - Pai, Man H.

AU - Wu, Ching Hsiang

AU - Liu, Jun J.

AU - Yeh, Sung Ling

PY - 2010/4

Y1 - 2010/4

N2 - Background & aims: Arginine (Arg) was shown to have immunomodulatory effect and inhibits advanced glycation end product (AGE) formation in in vitro studies. This study investigated the effects of dietary Arg supplementation on renal receptor of AGE (RAGE) expressions and oxidative damage in diabetic rats. Methods: There were 1 normal control (NC) group and 2 diabetic groups in this study. Rats in the NC group were fed with a chow diet. One diabetic group (DM) was fed a common semipurified diet, while the other diabetic group received a diet in which part of the casein was replaced by Arg (DM-Arg) for 8 wk. Diabetes was induced by an intraperitoneal injection of nicotinamide followed by streptozotocin. Rats with blood glucose levels exceeding 180 mg/dL were considered diabetic. Blood samples were collected at the baseline and 8 wk. Kidneys of the animals were harvested at the end of the study for further analysis. Results: Plasma fructosamine contents were significantly higher in the diabetic groups than in the NC group. The DM group had higher fructosamine than the DM-Arg group. Kidney nitrotyrosine concentrations and nuclear factor-κB p65 protein expressions were significantly lower in the DM-Arg group than in the DM group. The result of immunohistochemical staining also showed that the expressions of RAGE in the kidneys were significantly lower in the DM-Arg group than in the DM group. Conclusions: These results suggest that dietary Arg supplementation may decrease renal RAGE expressions and oxidative damage in rats with type 2 diabetes.

AB - Background & aims: Arginine (Arg) was shown to have immunomodulatory effect and inhibits advanced glycation end product (AGE) formation in in vitro studies. This study investigated the effects of dietary Arg supplementation on renal receptor of AGE (RAGE) expressions and oxidative damage in diabetic rats. Methods: There were 1 normal control (NC) group and 2 diabetic groups in this study. Rats in the NC group were fed with a chow diet. One diabetic group (DM) was fed a common semipurified diet, while the other diabetic group received a diet in which part of the casein was replaced by Arg (DM-Arg) for 8 wk. Diabetes was induced by an intraperitoneal injection of nicotinamide followed by streptozotocin. Rats with blood glucose levels exceeding 180 mg/dL were considered diabetic. Blood samples were collected at the baseline and 8 wk. Kidneys of the animals were harvested at the end of the study for further analysis. Results: Plasma fructosamine contents were significantly higher in the diabetic groups than in the NC group. The DM group had higher fructosamine than the DM-Arg group. Kidney nitrotyrosine concentrations and nuclear factor-κB p65 protein expressions were significantly lower in the DM-Arg group than in the DM group. The result of immunohistochemical staining also showed that the expressions of RAGE in the kidneys were significantly lower in the DM-Arg group than in the DM group. Conclusions: These results suggest that dietary Arg supplementation may decrease renal RAGE expressions and oxidative damage in rats with type 2 diabetes.

KW - Arginine

KW - Fructosamine

KW - Nitrotyrosine

KW - RAGE expression

KW - Type 2 diabetes

UR - http://www.scopus.com/inward/record.url?scp=77949659633&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77949659633&partnerID=8YFLogxK

U2 - 10.1016/j.eclnm.2010.02.001

DO - 10.1016/j.eclnm.2010.02.001

M3 - Article

VL - 5

JO - e-SPEN

JF - e-SPEN

SN - 2212-8263

IS - 2

ER -