Superoxide anion scavenge effect of querem glauca thunb. in whole blood of patients with ankylosing spondylitis

Shiow Yunn Sheu, Yang Hwei Tsuang, Feng Lin Hsu, Fung Jou Lu, Hsiich Ching Chiang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Nine phenolic compounds, catechin (1), epicatechin (2), gallocatechin (3), epigallocatechin (4), procyanidin B-4 (5), catechin-3-O-rhamnoside (6), rutin (7), querglanin (8) and isoquerglanin (9) were isolated from oak leaves (Quercus glauca Thunb. Fagaceae). the latter two (8, 9) were identified as new compounds. Several Quercus species have been used in folk medicine as an astringent for hemorrhoids and for treatment of inflammation, jaundice. tumor. In this study, these compounds were tested for scavenging effects of the Superoxide anion in the whole blood of patients with ankylosing spondylitis by means of an ultra-sensitive chemoluminescence (CL) analyzer and lucigenin amplification. The results showed that at a concentration of 2.3 × 10-5 M, isoquerglanin (9) displayed the strongest inhibition activity (73.55%), followed by querglanin (8) (68.81 %) and then gallocatechin (3) and epigallocatechin (4) (66.97 and 60.17% inhibition, respectively). In addition, the blood chemoluminescence (CL) level of patients with ankylosing spondylitis was inhibited by Superoxide dismutase (SOD) but not by catalase, suggesting that Superoxide anion is the major component of reactive oxygen species (ROS) involved in this assay system.

Original languageEnglish
Pages (from-to)307-315
Number of pages9
JournalAmerican Journal of Chinese Medicine
Volume25
Issue number3-4
Publication statusPublished - 1997
Externally publishedYes

ASJC Scopus subject areas

  • Complementary and alternative medicine

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