Super-paramagnetic iron oxide nanoparticles for use in extrapulmonary tuberculosis diagnosis

C. N. Lee, Y. M. Wang, Wen-FuThomas Lai, T. J. Chen, M. C. Yu, C. L. Fang, F. L. Yu, Yu-Hui Tsai, W. H S Chang, C. S. Zuo, P. F. Renshaw

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

E149-E157 The limited sensitivity of serological tests for mycobacterial antigens has encouraged the development of a nanoparticle probe specific for the extrapulmonary form of Mycobacterium tuberculosis (Mtb). We developed an innovative probe comprised of super-paramagnetic iron oxide (SPIO) nanoparticles conjugated with Mtb surface antibody (MtbsAb-nanoparticles) to provide ultrasensitive imaging of biomarkers involved in extrapulmonary Mtb infection. MtbsAb-nanoparticles were significantly conjugated with Mtb bacilli. The extent of contrast enhancement reduction on magnetic resonance imaging (MRI) for Mtb and human monocytic THP1 cells was proportional to the concentration of MtbsAb-nanoparticles. When MtbsAb-nanoparticles were intravenously injected into mice bearing Mtb granulomas, the granulomatous site showed a 14-fold greater reduction in signal intensity enhancement on T 2-weighted MR images compared with an opposing site that received PBS injection. Mtb sAb-nanoparticles represent a new non-invasive technology for the diagnosis of extrapulmonary Mtb.

Original languageEnglish
JournalClinical Microbiology and Infection
Volume18
Issue number6
DOIs
Publication statusPublished - Jun 2012

Fingerprint

Mycobacterium tuberculosis
Nanoparticles
Tuberculosis
Mycobacterium Infections
ferric oxide
Serologic Tests
Granuloma
Bacillus
Biomarkers
Magnetic Resonance Imaging
Technology
Antigens
Injections
Antibodies

Keywords

  • Diagnosis
  • Extrapulmonary
  • Magnetic resonance imaging
  • Mycobaterium tuberculosis
  • Nanoparticle

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Super-paramagnetic iron oxide nanoparticles for use in extrapulmonary tuberculosis diagnosis. / Lee, C. N.; Wang, Y. M.; Lai, Wen-FuThomas; Chen, T. J.; Yu, M. C.; Fang, C. L.; Yu, F. L.; Tsai, Yu-Hui; Chang, W. H S; Zuo, C. S.; Renshaw, P. F.

In: Clinical Microbiology and Infection, Vol. 18, No. 6, 06.2012.

Research output: Contribution to journalArticle

Lee, C. N. ; Wang, Y. M. ; Lai, Wen-FuThomas ; Chen, T. J. ; Yu, M. C. ; Fang, C. L. ; Yu, F. L. ; Tsai, Yu-Hui ; Chang, W. H S ; Zuo, C. S. ; Renshaw, P. F. / Super-paramagnetic iron oxide nanoparticles for use in extrapulmonary tuberculosis diagnosis. In: Clinical Microbiology and Infection. 2012 ; Vol. 18, No. 6.
@article{c5282d0992d246c893cd251d574ffffa,
title = "Super-paramagnetic iron oxide nanoparticles for use in extrapulmonary tuberculosis diagnosis",
abstract = "E149-E157 The limited sensitivity of serological tests for mycobacterial antigens has encouraged the development of a nanoparticle probe specific for the extrapulmonary form of Mycobacterium tuberculosis (Mtb). We developed an innovative probe comprised of super-paramagnetic iron oxide (SPIO) nanoparticles conjugated with Mtb surface antibody (MtbsAb-nanoparticles) to provide ultrasensitive imaging of biomarkers involved in extrapulmonary Mtb infection. MtbsAb-nanoparticles were significantly conjugated with Mtb bacilli. The extent of contrast enhancement reduction on magnetic resonance imaging (MRI) for Mtb and human monocytic THP1 cells was proportional to the concentration of MtbsAb-nanoparticles. When MtbsAb-nanoparticles were intravenously injected into mice bearing Mtb granulomas, the granulomatous site showed a 14-fold greater reduction in signal intensity enhancement on T 2-weighted MR images compared with an opposing site that received PBS injection. Mtb sAb-nanoparticles represent a new non-invasive technology for the diagnosis of extrapulmonary Mtb.",
keywords = "Diagnosis, Extrapulmonary, Magnetic resonance imaging, Mycobaterium tuberculosis, Nanoparticle",
author = "Lee, {C. N.} and Wang, {Y. M.} and Wen-FuThomas Lai and Chen, {T. J.} and Yu, {M. C.} and Fang, {C. L.} and Yu, {F. L.} and Yu-Hui Tsai and Chang, {W. H S} and Zuo, {C. S.} and Renshaw, {P. F.}",
year = "2012",
month = "6",
doi = "10.1111/j.1469-0691.2012.03809.x",
language = "English",
volume = "18",
journal = "Clinical Microbiology and Infection",
issn = "1198-743X",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Super-paramagnetic iron oxide nanoparticles for use in extrapulmonary tuberculosis diagnosis

AU - Lee, C. N.

AU - Wang, Y. M.

AU - Lai, Wen-FuThomas

AU - Chen, T. J.

AU - Yu, M. C.

AU - Fang, C. L.

AU - Yu, F. L.

AU - Tsai, Yu-Hui

AU - Chang, W. H S

AU - Zuo, C. S.

AU - Renshaw, P. F.

PY - 2012/6

Y1 - 2012/6

N2 - E149-E157 The limited sensitivity of serological tests for mycobacterial antigens has encouraged the development of a nanoparticle probe specific for the extrapulmonary form of Mycobacterium tuberculosis (Mtb). We developed an innovative probe comprised of super-paramagnetic iron oxide (SPIO) nanoparticles conjugated with Mtb surface antibody (MtbsAb-nanoparticles) to provide ultrasensitive imaging of biomarkers involved in extrapulmonary Mtb infection. MtbsAb-nanoparticles were significantly conjugated with Mtb bacilli. The extent of contrast enhancement reduction on magnetic resonance imaging (MRI) for Mtb and human monocytic THP1 cells was proportional to the concentration of MtbsAb-nanoparticles. When MtbsAb-nanoparticles were intravenously injected into mice bearing Mtb granulomas, the granulomatous site showed a 14-fold greater reduction in signal intensity enhancement on T 2-weighted MR images compared with an opposing site that received PBS injection. Mtb sAb-nanoparticles represent a new non-invasive technology for the diagnosis of extrapulmonary Mtb.

AB - E149-E157 The limited sensitivity of serological tests for mycobacterial antigens has encouraged the development of a nanoparticle probe specific for the extrapulmonary form of Mycobacterium tuberculosis (Mtb). We developed an innovative probe comprised of super-paramagnetic iron oxide (SPIO) nanoparticles conjugated with Mtb surface antibody (MtbsAb-nanoparticles) to provide ultrasensitive imaging of biomarkers involved in extrapulmonary Mtb infection. MtbsAb-nanoparticles were significantly conjugated with Mtb bacilli. The extent of contrast enhancement reduction on magnetic resonance imaging (MRI) for Mtb and human monocytic THP1 cells was proportional to the concentration of MtbsAb-nanoparticles. When MtbsAb-nanoparticles were intravenously injected into mice bearing Mtb granulomas, the granulomatous site showed a 14-fold greater reduction in signal intensity enhancement on T 2-weighted MR images compared with an opposing site that received PBS injection. Mtb sAb-nanoparticles represent a new non-invasive technology for the diagnosis of extrapulmonary Mtb.

KW - Diagnosis

KW - Extrapulmonary

KW - Magnetic resonance imaging

KW - Mycobaterium tuberculosis

KW - Nanoparticle

UR - http://www.scopus.com/inward/record.url?scp=84862785589&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862785589&partnerID=8YFLogxK

U2 - 10.1111/j.1469-0691.2012.03809.x

DO - 10.1111/j.1469-0691.2012.03809.x

M3 - Article

VL - 18

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

SN - 1198-743X

IS - 6

ER -