Super-paramagnetic iron oxide nanoparticles for use in extrapulmonary tuberculosis diagnosis

C. N. Lee; Y. M. Wang; Wen-FuThomas Lai; T. J. Chen; M. C. Yu; C. L. Fang; F. L. Yu; Yu-Hui Tsai; W. H S Chang; C. S. Zuo; P. F. Renshaw

doi:10.1111/j.1469-0691.2012.03809.x

Super-paramagnetic iron oxide nanoparticles for use in extrapulmonary tuberculosis diagnosis

C. N. Lee, Y. M. Wang, Wen-FuThomas Lai, T. J. Chen, M. C. Yu, C. L. Fang, F. L. Yu, Yu-Hui Tsai, W. H S Chang, C. S. Zuo, P. F. Renshaw

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

E149-E157 The limited sensitivity of serological tests for mycobacterial antigens has encouraged the development of a nanoparticle probe specific for the extrapulmonary form of Mycobacterium tuberculosis (Mtb). We developed an innovative probe comprised of super-paramagnetic iron oxide (SPIO) nanoparticles conjugated with Mtb surface antibody (MtbsAb-nanoparticles) to provide ultrasensitive imaging of biomarkers involved in extrapulmonary Mtb infection. MtbsAb-nanoparticles were significantly conjugated with Mtb bacilli. The extent of contrast enhancement reduction on magnetic resonance imaging (MRI) for Mtb and human monocytic THP1 cells was proportional to the concentration of MtbsAb-nanoparticles. When MtbsAb-nanoparticles were intravenously injected into mice bearing Mtb granulomas, the granulomatous site showed a 14-fold greater reduction in signal intensity enhancement on T ₂-weighted MR images compared with an opposing site that received PBS injection. Mtb sAb-nanoparticles represent a new non-invasive technology for the diagnosis of extrapulmonary Mtb.

Original language	English
Journal	Clinical Microbiology and Infection
Volume	18
Issue number	6
DOIs	https://doi.org/10.1111/j.1469-0691.2012.03809.x
Publication status	Published - Jun 2012

Keywords

Diagnosis
Extrapulmonary
Magnetic resonance imaging
Mycobaterium tuberculosis
Nanoparticle

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1469-0691.2012.03809.x

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abstract = "E149-E157 The limited sensitivity of serological tests for mycobacterial antigens has encouraged the development of a nanoparticle probe specific for the extrapulmonary form of Mycobacterium tuberculosis (Mtb). We developed an innovative probe comprised of super-paramagnetic iron oxide (SPIO) nanoparticles conjugated with Mtb surface antibody (MtbsAb-nanoparticles) to provide ultrasensitive imaging of biomarkers involved in extrapulmonary Mtb infection. MtbsAb-nanoparticles were significantly conjugated with Mtb bacilli. The extent of contrast enhancement reduction on magnetic resonance imaging (MRI) for Mtb and human monocytic THP1 cells was proportional to the concentration of MtbsAb-nanoparticles. When MtbsAb-nanoparticles were intravenously injected into mice bearing Mtb granulomas, the granulomatous site showed a 14-fold greater reduction in signal intensity enhancement on T 2-weighted MR images compared with an opposing site that received PBS injection. Mtb sAb-nanoparticles represent a new non-invasive technology for the diagnosis of extrapulmonary Mtb.",

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author = "Lee, {C. N.} and Wang, {Y. M.} and Wen-FuThomas Lai and Chen, {T. J.} and Yu, {M. C.} and Fang, {C. L.} and Yu, {F. L.} and Yu-Hui Tsai and Chang, {W. H S} and Zuo, {C. S.} and Renshaw, {P. F.}",

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AU - Lee, C. N.

AU - Wang, Y. M.

AU - Lai, Wen-FuThomas

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AU - Yu, M. C.

AU - Fang, C. L.

AU - Yu, F. L.

AU - Tsai, Yu-Hui

AU - Chang, W. H S

AU - Zuo, C. S.

AU - Renshaw, P. F.

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N2 - E149-E157 The limited sensitivity of serological tests for mycobacterial antigens has encouraged the development of a nanoparticle probe specific for the extrapulmonary form of Mycobacterium tuberculosis (Mtb). We developed an innovative probe comprised of super-paramagnetic iron oxide (SPIO) nanoparticles conjugated with Mtb surface antibody (MtbsAb-nanoparticles) to provide ultrasensitive imaging of biomarkers involved in extrapulmonary Mtb infection. MtbsAb-nanoparticles were significantly conjugated with Mtb bacilli. The extent of contrast enhancement reduction on magnetic resonance imaging (MRI) for Mtb and human monocytic THP1 cells was proportional to the concentration of MtbsAb-nanoparticles. When MtbsAb-nanoparticles were intravenously injected into mice bearing Mtb granulomas, the granulomatous site showed a 14-fold greater reduction in signal intensity enhancement on T 2-weighted MR images compared with an opposing site that received PBS injection. Mtb sAb-nanoparticles represent a new non-invasive technology for the diagnosis of extrapulmonary Mtb.

AB - E149-E157 The limited sensitivity of serological tests for mycobacterial antigens has encouraged the development of a nanoparticle probe specific for the extrapulmonary form of Mycobacterium tuberculosis (Mtb). We developed an innovative probe comprised of super-paramagnetic iron oxide (SPIO) nanoparticles conjugated with Mtb surface antibody (MtbsAb-nanoparticles) to provide ultrasensitive imaging of biomarkers involved in extrapulmonary Mtb infection. MtbsAb-nanoparticles were significantly conjugated with Mtb bacilli. The extent of contrast enhancement reduction on magnetic resonance imaging (MRI) for Mtb and human monocytic THP1 cells was proportional to the concentration of MtbsAb-nanoparticles. When MtbsAb-nanoparticles were intravenously injected into mice bearing Mtb granulomas, the granulomatous site showed a 14-fold greater reduction in signal intensity enhancement on T 2-weighted MR images compared with an opposing site that received PBS injection. Mtb sAb-nanoparticles represent a new non-invasive technology for the diagnosis of extrapulmonary Mtb.

KW - Diagnosis

KW - Extrapulmonary

KW - Magnetic resonance imaging

KW - Mycobaterium tuberculosis

KW - Nanoparticle

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Super-paramagnetic iron oxide nanoparticles for use in extrapulmonary tuberculosis diagnosis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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