99mtc-trodat-1 spect in healthy and 6-ohda lesioned parkinsonian monkeys

comparison with f-fdopa pet

W. S. Huang, K. H. MA, Y. H. Chou, C. Y. Chen, R. S. Liu, J. C. Liu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Parkinson's disease (PD) is a degeneration of the nigrostriatal dopaminergic pathway, leading to a selective loss of dopamine in the striatum. 99mTc-TRODAT-1 is a recently developed radiotracer that selectively binds to the dopamine transporters, which are significant because loss of these transporters corresponds with a loss of dopaminergic neurons. The present investigation compared 99mTc-TRODAT-1 single photon emission computed tomography (SPECT) with F-FDOPA positron emission tomography (PET) in the evaluation of PD using a primate model. Three monkeys, including one 6-hydroxydopamine lesioned PD model and two controls, were examined by both 99mTc-TRODAT-1 SPECT and F-FDOPA PET. For the PD monkey, expression of parkinsonian behaviour and F-FDOPA PET were used to evaluate the severity of the lesion. 99mTc-TRODAT-1 was prepared from a lyophilized kit. After intravenous injection of the radiotracer, SPECT was acquired over 4 h using a dual-head camera equipped with ultra-high resolution fan-beam collimators. Both uptake measurement and visual assessment were performed. Data were compared with motor behaviour and PET imaging. The striatal uptake in both healthy and PD monkeys increased continuously during the study, although the gradient of increase was less prominent in the diseased monkey. The increased uptake in the controls appeared to become blunt around 4 h after injection. A profound decrease of 99mTc-TRODAT-1 uptake was found in the striatum of the PD monkey compared with the controls (0.91 vs 2.16). In the PD monkey, the decrease of striatal uptake contralateral to the more affected side of the body was more prominent compared to the ipsilateral side (0.77 vs 1.06). In addition, greater loss occurred in the contralateral side of the putamen (0.54 vs 1.04). Changes of uptake ratios in the striatum and its subnuclei of the PD monkey were significantly correlated with those measured from PET. The loss of striatal uptake appeared greater in SPECT than the corresponding PET with both visual and uptake analyses. In conclusion, our data in a limited series of cases indicate that 99mTc-TRODAT-1 with a conventional nuclear medicine camera system may provide a suitable tool in evaluating parkinsonism in a primate model.

Original languageEnglish
Pages (from-to)77-83
Number of pages7
JournalNuclear Medicine Communications
Volume24
Issue number1
DOIs
Publication statusPublished - Jan 1 2003
Externally publishedYes

Fingerprint

Pets
Haplorhini
Parkinson Disease
Positron-Emission Tomography
Single-Photon Emission-Computed Tomography
Corpus Striatum
Primates
Monkey Diseases
Dopamine Plasma Membrane Transport Proteins
Gamma Cameras
Oxidopamine
Dopaminergic Neurons
Putamen
Nuclear Medicine
Parkinsonian Disorders
Intravenous Injections
technetium Tc 99m TRODAT-1
Dopamine
Head
Injections

Keywords

  • Tc-TRODAT-1 SPECT
  • F-FDOPA PET
  • Parkinsonian monkey

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

99mtc-trodat-1 spect in healthy and 6-ohda lesioned parkinsonian monkeys : comparison with f-fdopa pet. / Huang, W. S.; MA, K. H.; Chou, Y. H.; Chen, C. Y.; Liu, R. S.; Liu, J. C.

In: Nuclear Medicine Communications, Vol. 24, No. 1, 01.01.2003, p. 77-83.

Research output: Contribution to journalArticle

Huang, W. S. ; MA, K. H. ; Chou, Y. H. ; Chen, C. Y. ; Liu, R. S. ; Liu, J. C. / 99mtc-trodat-1 spect in healthy and 6-ohda lesioned parkinsonian monkeys : comparison with f-fdopa pet. In: Nuclear Medicine Communications. 2003 ; Vol. 24, No. 1. pp. 77-83.
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