@article{2926020813c04cb88229606e8dc96a78,
title = "1H NMR-based isolation of anti-inflammatory 9,11-secosteroids from the octocoral sinularia leptoclados",
abstract = "Octocoral Sinularia leptoclados has been identified as a source of bioactive 9,11-secosteroids. This study adopted a targeted isolation approach to the discovery and analysis of five 9,11-secosteroids, including two novel compounds named sinleptosterols A (1) and B (2) as well as five known analogues (8αH-3β,11-dihydroxy-24-methylene-9,11-secocholest-5-en-9-one (3), 8βH-3β,11-dihydroxy-24-methylene-9,11-secocholest-5-en-9-one (4), leptosterol A (5), (24S)-3β,11-dihydroxy-24-methyl-9,11-secocholest-5-en-9-one (6), and 3β,11-dihydroxy-9,11-secogorgost-5-en-9-one (7)) in terms of 1H-NMR patterns and potency against neutrophilic inflammation. The structure of secosteroids 1 and 2 was deduced from general spectroscopic analysis and an examination of NMR spectra. Among the above-mentioned isolates, compound 4 had the most pronounced effect in inhibiting elastase release and superoxide anion generation, with the IC50 values of 2.96 and 1.63 µM, respectively.",
keywords = "Antineutrophilic inflammation, Elastase, Secosteroids, Sinularia leptoclados, Superoxide anion",
author = "Chang, {Yu Chia} and Lai, {Kuei Hung} and Sunil Kumar and Chen, {Po Jen} and Wu, {Yi Hsuan} and Lai, {Ching Long} and Hsieh, {Hsi Lung} and Sung, {Ping Jyun} and Hwang, {Tsong Long}",
note = "Funding Information: Funding: This work was supported by grants from the Chang Gung Medical Research Foundation (BMRP450, CMRPG6J0141~3, CMRPF1G0241~3, CMRPF1H0051~53; CMRPF1I0041~2 and CMRPF1J0051~3), the Chang Gung University of Science and Technology (ZRRPF3H0101, ZRRPF3H0111, ZRRPF3H0131, and ZRRPF3J0081), Ministry of Education (EMRPD1K0421, EMRPD1K0481, EMRPD1I0441 and EMRPD1I0501), and the Ministry of Science and Technology (MOST 106-2320-B-255-003-MY3, 108-2320-B-255-003-MY3, 107-2320-B-182A-004-MY2, MOST107-2320-B-255-003; MOST108-2320-B-255-002-MY3 and 106-2320-B-291-001-MY3), Taiwan. Funding Information: This work was supported by grants from the Chang Gung Medical Research Foundation (BMRP450, CMRPG6J0141~3, CMRPF1G0241~3, CMRPF1H0051~53; CMRPF1I0041~2 and CMRPF1J0051~3), the Chang Gung University of Science and Technology (ZRRPF3H0101, ZRRPF3H0111, ZRRPF3H0131, and ZRRPF3J0081), Ministry of Education (EMRPD1K0421, EMRPD1K0481, EMRPD1I0441 and EMRPD1I0501), and the Ministry of Science and Technology (MOST 106-2320-B-255-003-MY3, 108-2320-B-255-003-MY3, 107-2320-B-182A-004-MY2, MOST107-2320-B-255-003; MOST108-2320-B-255-002-MY3 and 106-2320-B-291-001-MY3), Taiwan. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
month = may,
doi = "10.3390/md18050271",
language = "English",
volume = "18",
journal = "Marine Drugs",
issn = "1660-3397",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "5",
}