13C-NMR of the Deoxyribose Sugars in Four DNA Oligonucleotide Duplexes: Assignment and Structural Features

Steven R. LaPlante, Nilo Zanatta, Anna Hakkinen, Andrew H.J. Wang, Philip N. Borer

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Natural-abundance 13C-NMR spectra have been obtained for four self-complementary DNA oligonucleotides: [d(TAGCGCTA)]2, [d(GGTATACC)]2, [d(CG)3]2, and [d(TCGCG)]2; this paper focuses on the deoxyribose resonances. Assignments were made by a combination of the two-dimensional protondetected heteronuclear correlation experiment and comparison of 1D spectra, accounting for 31P coupling, base composition, and similarities in chemical shift versus temperature profiles (δ vs T). Large shielding and deshielding of the sugar resonances (between 2.0 and -1.9 ppm) are observed upon thermal dissociation of the duplex. The shapes of the δ vs T profiles correlate strongly with the purine/pyrimidine nature of the base attached at C1′ in these duplexes that have a substantial fraction of residues within alternating purine-pyrimidine sequences. The correlation is primarily associated with changes in the equilibrium distribution of furanose pseudorotational states that may arise in part from the relief of interstrand purine-purine steric clashes.

Original languageEnglish
Pages (from-to)2430-2440
Number of pages11
JournalBiochemistry
Volume33
Issue number9
DOIs
Publication statusPublished - Mar 1 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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