Sumoylation of Rta of Epstein-Barr virus is preferentially enhanced by PIASxβ

Shih Tung Liu, Wen Hung Wang, Yi Ren Hong, Jian Ying Chuang, Pei Jung Lu, Li Kwan Chang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) expresses an immediate-early protein, Rta, to activate the viral lytic cycle. This study identifies PIASxα and PIASxβ as binding partners of Rta in a yeast two-hybrid screen and demonstrates the binding of Rta to PIASxα and PIASxβ in vitro by GST pull-down analysis. Coimmunoprecipitation experiments and indirect immunofluorescence analysis show that Rta interacts and colocalizes with PIASxα and PIASxβ in the nucleus. These interactions seem to enhance Rta sumoylation as transfecting plasmids expressing PIASxα, PIASxβ, Ubc9, or SUMO-1 increase the capacity of Rta to transactivate a promoter that contains an Rta-response element and the promoters of p21 and BNLF1 in transient transfection assay. This study also finds that Rta sumoylation is preferentially enhanced by PIASxβ, which could be attributed to the fact that PIASxβ, compared to PIASxα, has a strong affinity to Rta, suggesting that affinity of a SUMO E3 ligase to its target protein influences the function of protein sumoylation.

Original languageEnglish
Pages (from-to)163-170
Number of pages8
JournalVirus Research
Volume119
Issue number2
DOIs
Publication statusPublished - Aug 2006
Externally publishedYes

Fingerprint

Sumoylation
Human Herpesvirus 4
Immediate-Early Proteins
Ubiquitin-Protein Ligases
Response Elements
Indirect Fluorescent Antibody Technique
Transfection
Proteins
Plasmids
Yeasts

Keywords

  • EBV
  • PIASxα
  • PIASxβ
  • Rta

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Cite this

Sumoylation of Rta of Epstein-Barr virus is preferentially enhanced by PIASxβ. / Liu, Shih Tung; Wang, Wen Hung; Hong, Yi Ren; Chuang, Jian Ying; Lu, Pei Jung; Chang, Li Kwan.

In: Virus Research, Vol. 119, No. 2, 08.2006, p. 163-170.

Research output: Contribution to journalArticle

Liu, Shih Tung ; Wang, Wen Hung ; Hong, Yi Ren ; Chuang, Jian Ying ; Lu, Pei Jung ; Chang, Li Kwan. / Sumoylation of Rta of Epstein-Barr virus is preferentially enhanced by PIASxβ. In: Virus Research. 2006 ; Vol. 119, No. 2. pp. 163-170.
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