Subcellular distributions of calcitonin gene-related peptide (CGRP)-like immunoreactivity in the subcommissural organ of the golden hamster (Mesocricetus auratus)

Mang Hung Tsai, Ching Hsiang Wu, Wen Pin Chen, Jeng Yung Shieh, Chen Yuan Wen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Immuno-electron microscopy specifically enhanced with silver staining has been used to demonstrate the localization of calcitonin gene-related peptide (CGRP) in the ependymocytes of the hamster subcommissural organ (SCO). Hamster SCO consists of the ependymal and hypendymal cell layers, the latter being arranged as rosette-like structure across the posterior commissure (PC) and often arranged with longitudinal axis parallel to the ventricle. All cytoplasmic regions of the ependymal and hypendymal cells were strongly stained with CGRP. In the hypendymal layer, the CGRP positive hypendymal cells were frequently in contact with local blood vessels and arranged in-groups traversing the thick portion of the PC. Ultrastructurally, the CGRP-immunoreaction products were distributed at the dilated cisternae of the rough endoplasmic reticulum (rER) and secretory granules of the ependymal and hypendymal cells. The dilated cisterna of the rER was usually concentrated in the basal region of the ependymal cells and irregular in shape. These dilated cisternae were filled with a flocculent material or finely granular substance, but hardly studded with ribosomes. Labelled secretory granules were abundant in the apical pole of the ependymal cells and discharged their contents into the third ventricle in the form of a thin layer of secretion. This CGRP positive material appeared to constitute the pre-Reissner's fiber (RF). On the basis of the present ultrastructural evidences, we proposed that ependymocytic CGRP in SCO may be synthesized and stored in the cisternae of rER, then released and incorporated into the RF in the third ventricle through the secretory granules. The abundant amount of CGRP in ependymocytes of SCO and RF in the third ventricle suggests a significant endocrine function of CGRP in hamster SCO.

Original languageEnglish
Pages (from-to)85-95
Number of pages11
JournalNeuroscience Research
Volume47
Issue number1
DOIs
Publication statusPublished - Sep 1 2003
Externally publishedYes

Fingerprint

Subcommissural Organ
Calcitonin Gene-Related Peptide
Mesocricetus
Third Ventricle
Rough Endoplasmic Reticulum
Secretory Vesicles
Cricetinae
Silver Staining
Immunoelectron Microscopy
Ribosomes
Blood Vessels

Keywords

  • Calcitonin gene-related peptide
  • Immunohisochemistry
  • Secretion release
  • Subcommissural organ

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Subcellular distributions of calcitonin gene-related peptide (CGRP)-like immunoreactivity in the subcommissural organ of the golden hamster (Mesocricetus auratus). / Tsai, Mang Hung; Wu, Ching Hsiang; Chen, Wen Pin; Shieh, Jeng Yung; Wen, Chen Yuan.

In: Neuroscience Research, Vol. 47, No. 1, 01.09.2003, p. 85-95.

Research output: Contribution to journalArticle

@article{08f365560d474bb992afb4e6e6eea3ca,
title = "Subcellular distributions of calcitonin gene-related peptide (CGRP)-like immunoreactivity in the subcommissural organ of the golden hamster (Mesocricetus auratus)",
abstract = "Immuno-electron microscopy specifically enhanced with silver staining has been used to demonstrate the localization of calcitonin gene-related peptide (CGRP) in the ependymocytes of the hamster subcommissural organ (SCO). Hamster SCO consists of the ependymal and hypendymal cell layers, the latter being arranged as rosette-like structure across the posterior commissure (PC) and often arranged with longitudinal axis parallel to the ventricle. All cytoplasmic regions of the ependymal and hypendymal cells were strongly stained with CGRP. In the hypendymal layer, the CGRP positive hypendymal cells were frequently in contact with local blood vessels and arranged in-groups traversing the thick portion of the PC. Ultrastructurally, the CGRP-immunoreaction products were distributed at the dilated cisternae of the rough endoplasmic reticulum (rER) and secretory granules of the ependymal and hypendymal cells. The dilated cisterna of the rER was usually concentrated in the basal region of the ependymal cells and irregular in shape. These dilated cisternae were filled with a flocculent material or finely granular substance, but hardly studded with ribosomes. Labelled secretory granules were abundant in the apical pole of the ependymal cells and discharged their contents into the third ventricle in the form of a thin layer of secretion. This CGRP positive material appeared to constitute the pre-Reissner's fiber (RF). On the basis of the present ultrastructural evidences, we proposed that ependymocytic CGRP in SCO may be synthesized and stored in the cisternae of rER, then released and incorporated into the RF in the third ventricle through the secretory granules. The abundant amount of CGRP in ependymocytes of SCO and RF in the third ventricle suggests a significant endocrine function of CGRP in hamster SCO.",
keywords = "Calcitonin gene-related peptide, Immunohisochemistry, Secretion release, Subcommissural organ",
author = "Tsai, {Mang Hung} and Wu, {Ching Hsiang} and Chen, {Wen Pin} and Shieh, {Jeng Yung} and Wen, {Chen Yuan}",
year = "2003",
month = "9",
day = "1",
doi = "10.1016/S0168-0102(03)00185-8",
language = "English",
volume = "47",
pages = "85--95",
journal = "Neuroscience Research",
issn = "0168-0102",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Subcellular distributions of calcitonin gene-related peptide (CGRP)-like immunoreactivity in the subcommissural organ of the golden hamster (Mesocricetus auratus)

AU - Tsai, Mang Hung

AU - Wu, Ching Hsiang

AU - Chen, Wen Pin

AU - Shieh, Jeng Yung

AU - Wen, Chen Yuan

PY - 2003/9/1

Y1 - 2003/9/1

N2 - Immuno-electron microscopy specifically enhanced with silver staining has been used to demonstrate the localization of calcitonin gene-related peptide (CGRP) in the ependymocytes of the hamster subcommissural organ (SCO). Hamster SCO consists of the ependymal and hypendymal cell layers, the latter being arranged as rosette-like structure across the posterior commissure (PC) and often arranged with longitudinal axis parallel to the ventricle. All cytoplasmic regions of the ependymal and hypendymal cells were strongly stained with CGRP. In the hypendymal layer, the CGRP positive hypendymal cells were frequently in contact with local blood vessels and arranged in-groups traversing the thick portion of the PC. Ultrastructurally, the CGRP-immunoreaction products were distributed at the dilated cisternae of the rough endoplasmic reticulum (rER) and secretory granules of the ependymal and hypendymal cells. The dilated cisterna of the rER was usually concentrated in the basal region of the ependymal cells and irregular in shape. These dilated cisternae were filled with a flocculent material or finely granular substance, but hardly studded with ribosomes. Labelled secretory granules were abundant in the apical pole of the ependymal cells and discharged their contents into the third ventricle in the form of a thin layer of secretion. This CGRP positive material appeared to constitute the pre-Reissner's fiber (RF). On the basis of the present ultrastructural evidences, we proposed that ependymocytic CGRP in SCO may be synthesized and stored in the cisternae of rER, then released and incorporated into the RF in the third ventricle through the secretory granules. The abundant amount of CGRP in ependymocytes of SCO and RF in the third ventricle suggests a significant endocrine function of CGRP in hamster SCO.

AB - Immuno-electron microscopy specifically enhanced with silver staining has been used to demonstrate the localization of calcitonin gene-related peptide (CGRP) in the ependymocytes of the hamster subcommissural organ (SCO). Hamster SCO consists of the ependymal and hypendymal cell layers, the latter being arranged as rosette-like structure across the posterior commissure (PC) and often arranged with longitudinal axis parallel to the ventricle. All cytoplasmic regions of the ependymal and hypendymal cells were strongly stained with CGRP. In the hypendymal layer, the CGRP positive hypendymal cells were frequently in contact with local blood vessels and arranged in-groups traversing the thick portion of the PC. Ultrastructurally, the CGRP-immunoreaction products were distributed at the dilated cisternae of the rough endoplasmic reticulum (rER) and secretory granules of the ependymal and hypendymal cells. The dilated cisterna of the rER was usually concentrated in the basal region of the ependymal cells and irregular in shape. These dilated cisternae were filled with a flocculent material or finely granular substance, but hardly studded with ribosomes. Labelled secretory granules were abundant in the apical pole of the ependymal cells and discharged their contents into the third ventricle in the form of a thin layer of secretion. This CGRP positive material appeared to constitute the pre-Reissner's fiber (RF). On the basis of the present ultrastructural evidences, we proposed that ependymocytic CGRP in SCO may be synthesized and stored in the cisternae of rER, then released and incorporated into the RF in the third ventricle through the secretory granules. The abundant amount of CGRP in ependymocytes of SCO and RF in the third ventricle suggests a significant endocrine function of CGRP in hamster SCO.

KW - Calcitonin gene-related peptide

KW - Immunohisochemistry

KW - Secretion release

KW - Subcommissural organ

UR - http://www.scopus.com/inward/record.url?scp=0041660938&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0041660938&partnerID=8YFLogxK

U2 - 10.1016/S0168-0102(03)00185-8

DO - 10.1016/S0168-0102(03)00185-8

M3 - Article

VL - 47

SP - 85

EP - 95

JO - Neuroscience Research

JF - Neuroscience Research

SN - 0168-0102

IS - 1

ER -