Study the property of double-ended fluoroalkyl poly(ethylene glycol) hydrogel as a depot for hydrophobic drug delivery using electron paramagnetic resonance technique and cell proliferation assay

Xiangli Liu, Yougang Mao, Errol V. Mathias, Collin Ma, Osmundo Franco, Yong Ba, Julia A. Kornfield, Tieli Wang, Lijun Xue, Bing Sen Zhou, Yun Yen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Hydrogel formed by fluoroalkyl double-ended polyethylene glycol (R f-PEG) micelles was studied to assess its properties to encapsulate a hydrophobic electron spin labeled drug, Chlorambucil-Tempol adduct (CT), and to control and sustain the drug release. The drug loaded hydrogel samples were characterized with variable-temperature dependent EPR experiment, and EPR theoretical lineshape analysis. It was found that CT molecules reside in the hydrophobic Rf-cores/IPDU shells of the Rf-PEG micelles and the maximum molecular-level loading capacity was estimated to be 18.8 mg per gram of the Rf-PEG. It has been known that Rf-PEG hydrogel with certain molecular masses for the fluoroalkyl group and the PEG chain shows properties of sol/gel phase coexistence and surface erosion, which represent the favorable condition for a pharmaceutical depot to control the kinetics of drug release. To evaluate the Rf-PEG's biocompatibility and kinetics of the drug release, a cell proliferation assay was carried out on human oropharyngeal carcinoma (KB) cells. The results show that R f-PEG is biocompatible and able to release CT to the cell media with a constant equilibrium concentration independent of the amount of CT loaded hydrogel.

Original languageEnglish
Pages (from-to)269-278
Number of pages10
JournalJournal of Sol-Gel Science and Technology
Volume45
Issue number3
DOIs
Publication statusPublished - Mar 1 2008
Externally publishedYes

Keywords

  • Chlorambucil
  • Controlled release
  • Electron paramagnetic resonance
  • Polymeric drug depot
  • Proliferation assay
  • Rf-PEG hydrogel
  • Tempol

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Biomaterials
  • Condensed Matter Physics
  • Materials Chemistry

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