In this paper, we are investigating the different cultured structures scale in micrometer and nanometer. The experiment have been fabricated four types aluminum substrate structures which included none-structure, micro-structure, nano-structure, and combined micro/nano structure to fulfill the research in osteoblast-like cell (MG 63). Finally we performed the sterilization method to deal with the patterns of alumina sheet product in the clean room. Meanwhile, we demonstrated the osteoblast-like cell (MG63) to culture on the thin film. Finally, we have been used MTT assay study the behavior of cultured osteoblast-like cell to obtain the different properties between none-structure, micro-structure, nano-structure and micro/nano structure for adhesion, elongation and proliferation.