Studies of photokilling of bacteria using titanium dioxide nanoparticles

Yang Hwei Tsuang, Jui Sheng Sun, Yu Chen Huang, Chung Hsin Lu, Walter Hong Shong Chang, Chien Che Wang

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

Metal pins used to apply skeletal traction or external fixation devices protruding through skin are susceptible to the increased incidence of pin site infection. In this work, we tried to establish the photokilling effects of titanium dioxide (TiO2) nanoparticles on an orthopedic implant with an in vitro study. In these photocatalytic experiments, aqueous TiO2 was added to the tested microorganism. The time effect of TiO2 photoactivation was evaluated, and the loss of viability of five different bacteria suspensions (Escherichia coli, Pseudomonas aeruginosa, Staphylococus aureus, Enterococcus hirae, and Bacteroides fragilis) was examined by the viable count procedure. The bactericidal effect of TiO2 nanoparticle-coated metal plates was also tested. The ultraviolet (UV) dosage used in this experiment did not affect the viability of bacteria, and all bacteria survived well in the absence of TiO2 nanoparticles. The survival curve of microorganisms in the presence of TiO2 nanoparticles showed that nearly complete killing was achieved after 50 min of UV illumination. The formation of bacterial colonies above the TiO2 nanoparticle-coated metal plates also decreased significantly. In this study, we clearly demonstrated the bactericidal effects of titanium dioxide nanoparticles. In the presence of UV light, the titanium dioxide nanoparticles can be applicable to medical facilities where the potential for infection should be controlled.

Original languageEnglish
Pages (from-to)167-174
Number of pages8
JournalArtificial Organs
Volume32
Issue number2
DOIs
Publication statusPublished - Feb 2008
Externally publishedYes

Fingerprint

Nanoparticles
Titanium dioxide
Bacteria
Metal Nanoparticles
Plate metal
Microbial Viability
Microorganisms
External Fixators
Bacteroides fragilis
Traction
Ultraviolet Rays
Infection
Lighting
Pseudomonas aeruginosa
Orthopedics
Suspensions
Metals
titanium dioxide
Escherichia coli
Skin

Keywords

  • Bactericidal effect
  • Nanoparticles
  • Photocatalysis
  • Titanium dioxide

ASJC Scopus subject areas

  • Biophysics

Cite this

Studies of photokilling of bacteria using titanium dioxide nanoparticles. / Tsuang, Yang Hwei; Sun, Jui Sheng; Huang, Yu Chen; Lu, Chung Hsin; Chang, Walter Hong Shong; Wang, Chien Che.

In: Artificial Organs, Vol. 32, No. 2, 02.2008, p. 167-174.

Research output: Contribution to journalArticle

Tsuang, Yang Hwei ; Sun, Jui Sheng ; Huang, Yu Chen ; Lu, Chung Hsin ; Chang, Walter Hong Shong ; Wang, Chien Che. / Studies of photokilling of bacteria using titanium dioxide nanoparticles. In: Artificial Organs. 2008 ; Vol. 32, No. 2. pp. 167-174.
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