Studies of lectin receptors of rat microglia in culture: Receptor distribution and internalization

C. H. Wu, S. T. Yeh, E. A. Ling

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The preset study examined the lectin labeling of diverse morphological forms of microglia in culture. Similar to amoeboid microglial cells in vivo, polymorphic microglia showed lectin labeling at their plasma membranes, as well as in a few cytoplasmic vesicles and vacuoles. This labeling pattern was observed in cultured microglia incubated with isolectin at 4°C for 30 min. Five minutes after the temperature was raised to 37°C, the surface lectin receptors appeared to be internalized, as shown by the occurrence of many subsurface lectin-labeled vesicles, vacuoles and tubule-like structures. With longer incubation (up to 1-2 h at 37°C), many lysosomes and a few trans-Golgi saccules and associated ly sosome-like structures became labeled. Concomitant with these changes was a reduction of lectin labeling at the plasma, with labeling having vanished in most of the cells after 1-2 h of incubation. By 24 h, only a few cells retained surface lectin labeling. It appears, therefore, that irrespective of morphology, lectin labeling (including its intracellular pathway) of microglia in culture parallels that of amoeboid microglia in vivo. This would offer a useful model for the study of lectin turnover in microglia and help to explain the roles of such receptors in microglial differentiation and function.

Original languageEnglish
Pages (from-to)89-99
Number of pages11
JournalExperimental Brain Research
Volume124
Issue number1
DOIs
Publication statusPublished - 1999

Fingerprint

Mitogen Receptors
Microglia
Lectins
Vacuoles
Cytoplasmic Vesicles
Saccule and Utricle
Lysosomes
Cell Membrane
Temperature

Keywords

  • Brain macrophages
  • Intracellular pathway
  • Isolectin
  • Microglial culture
  • Ultrastructure

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Studies of lectin receptors of rat microglia in culture : Receptor distribution and internalization. / Wu, C. H.; Yeh, S. T.; Ling, E. A.

In: Experimental Brain Research, Vol. 124, No. 1, 1999, p. 89-99.

Research output: Contribution to journalArticle

@article{3b1c44078e9149faa2202e6bdc27bced,
title = "Studies of lectin receptors of rat microglia in culture: Receptor distribution and internalization",
abstract = "The preset study examined the lectin labeling of diverse morphological forms of microglia in culture. Similar to amoeboid microglial cells in vivo, polymorphic microglia showed lectin labeling at their plasma membranes, as well as in a few cytoplasmic vesicles and vacuoles. This labeling pattern was observed in cultured microglia incubated with isolectin at 4°C for 30 min. Five minutes after the temperature was raised to 37°C, the surface lectin receptors appeared to be internalized, as shown by the occurrence of many subsurface lectin-labeled vesicles, vacuoles and tubule-like structures. With longer incubation (up to 1-2 h at 37°C), many lysosomes and a few trans-Golgi saccules and associated ly sosome-like structures became labeled. Concomitant with these changes was a reduction of lectin labeling at the plasma, with labeling having vanished in most of the cells after 1-2 h of incubation. By 24 h, only a few cells retained surface lectin labeling. It appears, therefore, that irrespective of morphology, lectin labeling (including its intracellular pathway) of microglia in culture parallels that of amoeboid microglia in vivo. This would offer a useful model for the study of lectin turnover in microglia and help to explain the roles of such receptors in microglial differentiation and function.",
keywords = "Brain macrophages, Intracellular pathway, Isolectin, Microglial culture, Ultrastructure",
author = "Wu, {C. H.} and Yeh, {S. T.} and Ling, {E. A.}",
year = "1999",
doi = "10.1007/s002210050603",
language = "English",
volume = "124",
pages = "89--99",
journal = "Experimental Brain Research",
issn = "0014-4819",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Studies of lectin receptors of rat microglia in culture

T2 - Receptor distribution and internalization

AU - Wu, C. H.

AU - Yeh, S. T.

AU - Ling, E. A.

PY - 1999

Y1 - 1999

N2 - The preset study examined the lectin labeling of diverse morphological forms of microglia in culture. Similar to amoeboid microglial cells in vivo, polymorphic microglia showed lectin labeling at their plasma membranes, as well as in a few cytoplasmic vesicles and vacuoles. This labeling pattern was observed in cultured microglia incubated with isolectin at 4°C for 30 min. Five minutes after the temperature was raised to 37°C, the surface lectin receptors appeared to be internalized, as shown by the occurrence of many subsurface lectin-labeled vesicles, vacuoles and tubule-like structures. With longer incubation (up to 1-2 h at 37°C), many lysosomes and a few trans-Golgi saccules and associated ly sosome-like structures became labeled. Concomitant with these changes was a reduction of lectin labeling at the plasma, with labeling having vanished in most of the cells after 1-2 h of incubation. By 24 h, only a few cells retained surface lectin labeling. It appears, therefore, that irrespective of morphology, lectin labeling (including its intracellular pathway) of microglia in culture parallels that of amoeboid microglia in vivo. This would offer a useful model for the study of lectin turnover in microglia and help to explain the roles of such receptors in microglial differentiation and function.

AB - The preset study examined the lectin labeling of diverse morphological forms of microglia in culture. Similar to amoeboid microglial cells in vivo, polymorphic microglia showed lectin labeling at their plasma membranes, as well as in a few cytoplasmic vesicles and vacuoles. This labeling pattern was observed in cultured microglia incubated with isolectin at 4°C for 30 min. Five minutes after the temperature was raised to 37°C, the surface lectin receptors appeared to be internalized, as shown by the occurrence of many subsurface lectin-labeled vesicles, vacuoles and tubule-like structures. With longer incubation (up to 1-2 h at 37°C), many lysosomes and a few trans-Golgi saccules and associated ly sosome-like structures became labeled. Concomitant with these changes was a reduction of lectin labeling at the plasma, with labeling having vanished in most of the cells after 1-2 h of incubation. By 24 h, only a few cells retained surface lectin labeling. It appears, therefore, that irrespective of morphology, lectin labeling (including its intracellular pathway) of microglia in culture parallels that of amoeboid microglia in vivo. This would offer a useful model for the study of lectin turnover in microglia and help to explain the roles of such receptors in microglial differentiation and function.

KW - Brain macrophages

KW - Intracellular pathway

KW - Isolectin

KW - Microglial culture

KW - Ultrastructure

UR - http://www.scopus.com/inward/record.url?scp=0033031304&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033031304&partnerID=8YFLogxK

U2 - 10.1007/s002210050603

DO - 10.1007/s002210050603

M3 - Article

C2 - 9928793

AN - SCOPUS:0033031304

VL - 124

SP - 89

EP - 99

JO - Experimental Brain Research

JF - Experimental Brain Research

SN - 0014-4819

IS - 1

ER -