Structural compositions and biological activities of cell wall polysaccharides in the rhizome, stem, and leaf of Polygonatum odoratum (Mill.) Druce

Jing Li, Shih Yi Hsiung, Mu Rong Kao, Xiaohui Xing, Shu Chieh Chang, Damao Wang, Pei Yun Hsieh, Pi Hui Liang, Zaibiao Zhu, Ting Jen Rachel Cheng, Jiun Jie Shie, Jing Ping Liou, D. Wade Abbott, Sung Won Kwon, Yves S.Y. Hsieh

Research output: Contribution to journalArticlepeer-review

Abstract

Polygonatum odoratum is a perennial rhizomatous medicinal plant and different plant parts have been used in the treatment of various ailments. Herein, we have investigated the structural compositions of rhizome, leaf, and stem cell walls. We found 30–44% of polysaccharides in these wall preparations were cyclohexanediaminetetraacetic acid (CDTA) extractable, the proportion of heteromannans (HMs) in the rhizome is nearly three-fold compared to that of the leave and stem. The pectic polysaccharides of the rhizome are also structurally more diverse, with arabinans and type I and type II arabinogalactans being richest as shown by linkage study of the sodium carbonate (Na2CO3) extract. In addition, the 2-linked Araf was rhizome-specific, suggesting the cell walls in the rhizome had adapted to a more complex structure compared to that of the leaf and stem. Water-soluble polysaccharide fractions were also investigated, high proportion of Man as in 4-linked Manp indicated high proportion of HMs. The 21.4 kDa pectic polysaccharides and HMs derived from rhizome cell walls induced specific immune response in mice macrophage cells producing IL-1α and hematopoietic growth factors GM-CSF and G-CSF in vitro.

Original languageEnglish
Article number108662
JournalCarbohydrate Research
Volume521
DOIs
Publication statusPublished - Nov 2022

Keywords

  • GC-MS
  • HPAEC-PAD
  • Linkage analysis
  • MALDI-TOF-MS
  • Polygonatum odoratum
  • Polysaccharides

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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